Christian J. Pike ^a,*, Jenna C. Carroll^a,b, Emily R. Rosario ^a, Anna M. Barron ^a

^a Davis School of Gerontology, University of Southern California, Los Angeles, CA 90089, USA b Neuroscience Graduate Program, University of Southern California, Los Angeles, CA 90089, USA.

^* Corresponding author. Fax: +1 213 740 4787.

E-mail address: [email protected] (C.J. Pike).

Abstract

Risk for Alzheimer’s disease (AD) is associated with age-related loss of sex steroid hormones in both women and men. In post-menopausal women, the precipitous depletion of estrogens and progestogens is hypothesized to increase susceptibility to AD pathogenesis, a concept largely supported by epidemiological evidence but refuted by some clinical findings. Experimental evidence suggests that estrogens have numerous neuroprotective actions relevant to prevention of AD, in particular promotion of neuron viability and reduction of β-amyloid accumulation, a critical factor in the initiation and progression of AD. Recent findings suggest neural responsiveness to estrogen can diminish with age, reducing neuroprotective actions of estrogen and, consequently, potentially limiting the utility of hormone therapies in aged women. In addition, estrogen neuroprotective actions are also modulated by progestogens. Specifically, continuous progestogen exposure is associated with inhibition of estrogen actions whereas cyclic delivery of progestogens may enhance neural benefits of estrogen. In recent years, emerging literature has begun to elucidate a parallel relationship of sex steroid hormones and AD risk in men. Normal age-related testosterone loss in men is associated with increased risk to several diseases including AD. Like estrogen, testosterone has been established as an endogenous neuroprotective factor that not only increases neuronal resilience against AD-related insults, but also reduces β-amyloid accumulation. Androgen neuroprotective effects are mediated both directly by activation of androgen pathways and indirectly by aromatization to estradiol and initiation of protective estrogen signaling mechanisms. The successful use of hormone therapies in aging men and women to delay, prevent, and or treat AD will require additional research to optimize key parameters of hormone therapy and may benefit from the continuing development of selective estrogen and androgen receptor modulators.

Article history: Available online 7 May 2009.

Keywords: Estrogen; Testosterone; Progesterone; Alzheimer’s disease; b-Amyloid; Neuroprotection/

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Introduction

As expertly described in the accompanying review articles, sex steroid hormones are potent regulators of neuron survival in multiple CNS regions and across a variety of circumstances ranging from normal development to neural injury. A compelling, and as yet largely unrealized, promise of sex steroid hormones is the translation of their neuroprotective properties into efficacious strategies for the treatment and or prevention of age-related neurodegenerative disorders such as Alzheimer’s disease (AD). Despite this unfulfilled therapeutic potential, abundant experimental, epidemiological and clinical evidence suggest that neural actions androgens, estrogens, and perhaps even progestogens can reduce the risk for AD.

AD is an age-related neurological disease that is the leading cause of dementia. Neuropathologically, AD is characterized by brain region-specific deposition of β-amyloid protein (Aβ) which creates senile plaques, hyperphosphorylation of the cytoskeletal protein tau that forms lesions called neurofibrillary tangles and neuropil threads, glial activation which is associated with inflammatory responses, and both synaptic and neuronal loss [3,37,139,140,196]. Although the mechanisms of AD pathogenesis remain to be fully resolved, the leading hypothesis posits that the disease is initiated and driven by prolonged elevation of Aβ levels [140]. Aβ is a proteolytic byproduct of the metabolism of amyloid precursor protein, a widely expressed protein with numerous functions ranging from axonal transport to gene transcription [336]. As a consequence of amyloid precursor protein expression, Aβ is normally found as a soluble protein at low levels in fluids and tissues throughout the body. In theory, alterations in either the production or clearance of Aβ that sway Aβ homeostasis towards increased neural levels will promote the development of AD [140]. The accumulation of Aβ encourages its abnormal assembly into oligomeric species that exhibit an altered structural conformation and can induce a range of neurodegenerative effects [136]. Consequently, enormous effort has been expended on identifying factors that regulate Aβ accumulation and or affect its neurodegenerative properties. One such class of factors is sex steroid hormones.

In this review, we will discuss the neuroprotective properties of sex steroid hormones as they relate to AD pathogenesis, focusing largely on their effects on Aβ accumulation and its associated neurodegeneration. Estrogens are the most thoroughly studied steroid hormones in terms of AD. We will cover the epidemiological and clinical evidence that suggests depletion of ovarian hormones at menopause increases the risk of AD in post-menopausal women, a danger some studies suggest may be mitigated by estrogen-based hormone therapy (HT). Consistent with a protective role against AD, experimental studies demonstrate that estrogens not only reduce neuron loss induced by AD-related insults but also act to reduce Aβ levels. However, in the Women’s Health Initiative (WHI) trial, the most exhaustive clinical evaluation of HT thus far, HT was associated with increased rather than decreased risk of AD. Analysis of experimental work reveals several key limitations of estrogen’s neuroprotective actions that may contribute to this clinical observation, including loss of neural estrogen responsiveness with age and interactions with progestogens that may limit estrogen neuroprotection. A more recent and still emerging literature suggests a parallel relationship in men with their primary sex steroid hormone, testosterone. That is, normal age-related testosterone is associated with increased risk of AD in aging men. Like estrogens, androgens also exert neuroprotective properties relevant to AD, including promotion of survival in neurons challenged with AD-related insults and reduction of Aβ levels. Finally, we consider future directions in this field, emphasizing the clinical potential of sex steroid hormones in prevention rather than treatment of AD and the emerging promise of selective estrogen receptor and androgen receptor modulators.

2. Menopause, hormone therapy, and Alzheimer’s disease

Converging lines of evidence indicate a potentially important role of estrogens in regulating AD pathogenesis. Preliminary clues suggesting this possibility stemmed from reports of sex differences in AD risk, with women showing higher prevalence and incidence. Although sex differences in AD are difficult to interpret due to gender differences in life expectancy, many studies of various cohorts indicate that women are at greater risk of AD [9,17,39,93,101, 137,180,221,281,290]. Further, there is some evidence that AD pathogenesis may be more severe in women as indicated by sex differences in cognitive deficits and neuropathology [21,49,66,150], although other studies indicate men have higher levels of tau pathology [293,294]. Further, there is a stronger association between the apolipoprotein E ε4 allele and sporadic AD in women compared to men [66,84,165] and the ε4 allele has been shown to be associated with greater hippocampal atrophy and memory impairments in women compared to men [98]. When considered together, these epidemiological and neuropathological studies indicate sex differences in AD, suggesting that women may be more vulnerable to AD than men.

Several transgenic mouse models exhibit sex differences in AD-like neuropathology that appears to parallel that observed in human AD cases. For example, at both 15 and 19 mo of age, female Tg2576 mice display a higher plaque load burden and higher levels of both soluble and insoluble Aβ40 and Aβ42 than age-matched males [51]. Similarly, female APP_swexPS1 transgenic mice have higher Aβ load burden and plaque number than age-matched males [350]. The same pattern of greater Aβ deposition in female versus male mice is also observed in the 3xTg-AD triple transgenic mouse [156]. These studies in transgenic mouse models of AD suggest that the female brain may be more vulnerable to AD pathogenesis.

The increased risk of AD in women is presumed to be associated with the precipitous loss of estrogens and progesterone at menopause. Consistent with this position, plasma levels of the estrogen 17β-estradiol (E₂) [212] are reported to be lower in women with AD in comparison to age-matched controls. If the depletion of ovarian hormones at menopause contributes to women’s increased risk of AD, then one would predict that estrogen-based hormone therapy (HT) would be effective in the prevention and or treatment of AD. This critical issue remains unresolved with persuasive arguments both for and against the use of HT for AD. An early study of this issue found that AD risk was lower in women who used HT relative to nonusers and this risk decreased significantly as both dose and duration of HT use increased [247]. Similarly, findings from several other case control and prospective studies suggest that post-menopausal women with a history of HT use were at reduced risk of AD [52,151,184,248,329,351,382]. Further, a meta-analysis of studies found that HT was associated with decreased risk of cognitive dysfunction [158,197,241,305]. Collectively, these studies suggest a potential protective role of estrogen against the development of AD.

Despite indications of benefits, the potential protective of HT against AD remains controversial. Arguing against a protective role, several studies found that HT use was not associated with reduced risk of AD (reviewed in [146]) or failed to yield significant cognitive benefits [7,22,34,40,119,152,228,266,349,369]. One possible explanation for this discrepancy is suggested by findings from the Cache County Study, which demonstrated that the association between HT use and reduced risk of AD was strengthened in long-term HT users [382]. Interpretations of these findings include the concept that HT may have a largely preventive role against AD and or the hypothesis that early initiation of HT is essential as women who took HT for longer periods likely began treatment nearer the time of menopause.

The notion that estrogen-based HT can effectively reduce the risk of AD and improve age-related deficits in cognition has been challenged by findings from the Women’s Health Initiative Memory Study (WHIMS). WHIMS was a randomized, multi-center, double-blind, placebo-controlled study of ~4500 women between 65 and 79 yrs of age that evaluated effects of HT consisting of conjugated equine estrogen (CEE) alone or CEE with the progestin medroxyprogesterone acetate (MPA). This study reported that neither CEE nor CEE + MPA significantly improved cognition versus placebo in women showing cognitive decline associated with normal aging [91,273] or dementia [312,311]. In the CEE alone arm, there was no significant difference in dementia incidence between HT or placebo groups although there was a non-significant trend towards increased risk in the HT group [91,312]. In the CEE + MPA arm, they found that women receiving HT had a higher risk of probable dementia [311]. HT use was also associated with increased incidence of stroke and breast cancer, suggesting that the risks of HT may outweigh its benefits. Further, the important differences between the CEE alone arm and the CEE + MPA arm raise several issues about the inclusion of a progestin component.

Although the WHIMS findings raise serious concerns over HT use, many challenge the interpretation that these data dismiss the potential efficacy of HT in reducing the risk of AD [75,118,149,209,265,276]. A variety of issues have been identified that may have affected HT outcomes in the WHIMS compared to the many observational studies such as differences in methodological techniques, outcome measures, hormone exposures, menopausal symptoms, and the timing of hormone use [149]. In particular, neural sensitivity to sex steroid hormones may diminish during the menopause transition, resulting in a critical window in which to initiate HT in order to realize benefits (reviewed in [75]). Because HT was initiated many years after menopause in the WHIMS study, the study’s design may have inadvertently focused on an age group in which estrogen is minimally active in brain and thus was unlikely to detect potential cognitive benefits. In addition, as suggested by prior epidemiological and clinical find ings, estrogen may be most effective in preventing rather than treating AD. In this case, the relatively advanced age of WHIMS subjects would also be biased against positive outcomes. Additional issues include HT formulation, route of administration, and treatment regime (e.g., cyclic versus continuous hormone delivery). In order to unravel this conundrum, a greater understanding of the neuroprotective actions of estrogens and progestogens are needed, as well as their limitations in the context of aging.

In the following sections, we examine the neuroprotective effects of estrogen, focusing on its abilities to increase neuronal resilience against AD insults and to antagonize AD pathogenesis by reducing Aβ accumulation. Importantly, we also discuss experimental evidence that addresses how these protective actions of estrogen are affected by the concerns raised by WHIMS.

3. Estrogen neuroprotection and Alzheimer’s disease insults.

An established neural action of estrogens that may contribute to a protective role against AD is promotion of neuron viability. Estrogen is neuroprotective against a variety of insults in several cell culture and rodent paradigms of injury and neurodegenerative disease. Of particular interest is the ability of estrogen to protect against neuronal loss induced by Aβ, which is thought to be primary neurodegenerative agent in AD. Reports from several groups demonstrate that estrogen can protect cultured neurons and neural cell lines from Aβ mediated toxicity [26,124,132,135,222,259].

Estrogen may potentially protect against Aβ-induced neurotoxicity at several steps in the degenerative process. The leading theory of Aβ toxicity posits a pathologic assembly of Aβ involving adoption of a p-sheet conformation, resulting in a change in protein structure that is associated with a toxic gain of function (reviewed in [347]). Consistent with this working hypothesis of Aβ neurotoxicity, our prior work has shown that Aβ is toxic only in an assembled state [263,261]. Assembled Aβ in the form of soluble oligomers and insoluble fibrils can induce neuronal death [72,348,373] degeneration of neurites [169,262] and synaptic dys- ruption [145,182,288,348] leading to impaired learning and memory [61,198]. Interaction of Aβ assemblies with neurons initiates a cascade of upstream signaling mechanisms associated with cell death, including calcium dysregulation [216,356], oxidative stress [25,123,214], and activation of pro-inflammatory pathways promoting chronic gliosis [89,264]. Most evidence suggests that the plethora of upstream signaling cascades elicited by Aβ ultimately mediate neurotoxicity by downstream activation of neuronal apoptosis pathways [71,73]. In particular, we find that Aβ-induced neuronal apoptosis involves activation of JNK signaling and consequent dysregulation of the Bcl-2 family of apoptosis-related proteins [374].

Since neuronal apoptosis is an important downstream mediator of Aβ neurotoxicity, regulation of apoptosis is predicted to be a key mechanism of estrogen protection from Aβ. Consistent with this hypothesis, estrogen has been implicated in the regulation of Bcl- 2 family members in neurons [86,110,191,234,254,259,316,320]. The Bcl-2 family includes both proteins that promote cell survival (e.g., Bcl-2, Bcl-x_L, and Bcl-w) and others that antagonize it (e.g., Bax, Bad, Bak, Bik, Bid, BNIP3, and Bim) (reviewed in [12,70]). We have found that physiological levels of E₂ inhibit neuronal apoptosis at least in part by increasing expression of anti-apoptotic Bcl-x_L [259,320] and Bcl-w [375] while down-regulating expression of the pro-apoptotic Bim [375]. The observed effects of E₂ on Aβ mediated apoptosis were found to be mediated ER-dependent mechanisms, since the anti-apoptotic effects of E₂ were blocked by pre-treatment with an ER antagonist [259, 375]. Supporting this, it has been previously demonstrated that both ERα and ERβ are crucial in regulating Bcl-2 expression and neuronal survival [388] and recently it has been shown that E₂ can also increase Bcl-2 through Akt-dependent CREB activation [381].

Interestingly, estrogen dependent regulation of the Bcl-2 family of proteins has also been implicated in neuroprotection against excitotoxicity, a form of neuronal injury implicated in AD neurodegeneration (reviewed in [29,280]). Evidence suggests that in AD, Aβ toxicity and glutamate excitotoxicity may cooperatively activate pathways leading neuronal death. Glutamate-induced excitotoxic injury is potentiated by Aβ. In cell culture paradigms, the combination of sub-lethal concentrations of glutamate combined with sub-lethal levels Aβ yields robust neuronal loss [189,215]. Such degenerative interactions are predicted to occur in AD because the AD brain exhibits both Aβ accumulation and evidence of glutamate injury. Whether upstream and or downstream pathways of Aβ and glutamate action are responsible for their synergistic toxic effects is unclear. Glutamate excitotoxicity leads to calcium dysregulation and oxidative stress, and excitotoxic neuron death is mediated in part by apoptosis (reviewed in [64]).

Several studies have demonstrated that estrogen reduces excito- toxic neuronal death induced by glutamate agonists in cell culture [43,236,275,317,315,319]. For example, Dorsa and colleagues found that estrogen inhibited neuronal death in murine cortical cultures following excitotoxic insult, an effect that could be pharmacologically blocked with the ER antagonist, tamoxifen [315]. Further, Brin- ton and collegues found estrogen to promote intracellular Ca²⁺ accumulation in neuronal cultures treated glutamate at physiological doses, while inhibiting intracellular Ca²⁺ accumulation following treatment with excitotoxic glutamate doses [236]. Similar observations of estrogen neuroprotection following excitotoxic challenge have been reported by several groups [124,275,354].

Estrogen has also been shown to regulate the extent of excitotoxic injury in rodent models [15,14,55,286]. For example, administration of exogenous E₂ to ovariectomized (OVX) rats has been reported to protect against kainate-induced neuronal loss [14]. Additionally, depletion of endogenous estrogen levels may increase susceptibility to excitotoxicity, with pronounced kainate-induced neural loss observed in intact rats during proestrus or following OVX [15]. Similarly, we observe neuroprotection against kainate lesion following administration of estrogen to OVX rats [55,286].

Interestingly, estrogen neuroprotection against excitotoxic injury shares mechanistic similarities with protection against Aβ-in- duced apoptosis. That is, estrogen regulation of the Bcl-2 family is implicated in protective actions against glutamate-related injury [232,234,316,388]. Brinton and colleagues found that estrogen mediated neuroprotection against glutamate excitotoxicity by promoting mitochondrial Ca²⁺ sequestration, and this was associated with increased expression of Bcl-2 [232]. Estrogen dependent modulation Bcl-2 following excitotoxicity may be mediated by rapid, non-genomic ER-dependent signaling mechanisms [387,388]. Estrogen activates an ER-dependent Src/ERK/CREB signaling pathway that leads to upreguation of Bcl-2 [364]. In contrast, others suggest that estrogen may regulate Bcl-2 family expression through a direct genomic mechanism. Supporting this we have described an estrogen responsive element (ERE) on the Bcl-x gene [259], while others describe EREs on Bcl-2 [83,256]. These pathways are summarized in Fig. 1.

Figure 1. Estrogens activate neuroprotective pathways that may attenuate Alzheimer’s disease. Estrogens including 17β-estradiol (E₂) reduce neuronal apoptosis by (i) nongenomic signaling cascades, including activation of PI3 K, protein kinase C (PKC) and Src/ERK pathways, and (ii) genomic pathways utilizing CREB response elements (CRE) and estrogen response elements (ERE) on members of the Bcl-2 family of genes including Bcl-2, Bcl-x, Bcl-w, and Bim. Similarly, estrogens decrease levels of the AD-related protein Ab by (i) non-genomic signaling that promotes non-amyloidogenic processing of the Ab precursor protein (APP), and perhaps (ii) classic genomic mechanisms that may involve ERE, CRE, and or other steroid response elements (SRE) on the Ab-catabolizing enzymes neprilysin (NEP) and insulin degrading enzyme (IDE).

In addition to regulation of the Bcl-2 family of proteins, estrogen has also been implicated in neuroprotection against many other prominent features of the AD-neurodegenerative cascade including inflammation and oxidative stress. Many studies indicate that Aβ may be contribute to AD-related oxidative stress [25,123,214] and deposition of Aβ may activate microglia and promote inflammation [291,338]. Abundant evidence indicates that estrogen is a potent inhibitor of oxidative damage [340] and hydrogen peroxide mediated neuronal death [27,26,124]. Estrogen mediates these antioxi-dant effects through ER-independent mechanisms, acting as a free radical scavenger owing to its phenolic structure [279,278,324, 325]. However, these neuroprotective effects are only elicited at supraphysiological estrogen dosages [27,202], thereby limiting the clinical relevance of these neuroprotective actions of estrogens. In contrast, the anti-inflammatory actions of estrogen are mediated by ER-dependent mechanisms and may provide preventative or therapeutic benefit to a range of neurodegenerative diseases where neuroinflammation is a major degenerative process. Pluripotent effects of estrogen have been described on glial function and activation. Estrogen may suppress reactive gliotic responses associated with traumatic injury and neurodegeneration [109,111], while also promoting the neuroprotective properties of astrocytes by increasing arborization and promoting synaptogenesis [80,323]. Interestingly, estrogen has been found to attenuate microglial activation only when estrogen treatment was given to the cultures prior to inflammatory insult, indicating estrogen does not have the capacity to modulate inflammatory reactions once microglial activation has been initiated [341]. This suggests the anti-inflammatory benefits of estrogen may be limited to prevention of AD rather than treatment.

Another potentially significant neuroprotective action of estrogen that is highly relevant to AD and related neurodegenerative disorders is inhibition of pathological tau hyperphosphorylation. Both estrogen and progesterone can modulate activities of kinases and phosphatases involved in regulating levels of tau phosphorylation. Specifically, E₂ and P4 regulate tau phosphorylation through the glycogen synthase kinase-3β (GSK-3β) pathway [8,122]. Estrogen can reduce GSK-3β activity [122], and progesterone can decrease expression of both tau and GSK-3β. Estrogen can also lower tau hyperphosphorylation through the wnt signaling pathway and a gene called dickkopf-1 [386] as well as through the protein kinase A pathway [204]. These results demonstrate some of the first insights into the mechanism behind estrogen neuroprotection in tau-related disorders.

The described neuroprotective actions of estrogen against AD- related insults are largely mediated by activation of estrogen receptors (ER). It has been well established that both ER subtypes are widely distributed in the brain, including in brain regions affected in AD such as the hippocampus, frontal cortex, and amygdala [144,310,309]. Recently, changes in the subcellular distribution of ERs in hippocampal neurons have been implicated in AD pathogenesis [205]. Specifically, the shift of ERα from the nucleus to the cytoplasm may decrease the development of AD pathology in humans [155] and transgenic mice [181]. In addition, recent studies have demonstrated that ERα levels in the frontal cortex correlated with mini-mental state examination scores in women with end-stage AD [185] and that specific allele differences in ERα are correlated with an increased risk for AD in women with Down syndrome [296]. Also, ERβ immunoreactivity is reportedly increased in the hippocampus compared to age-matched controls [292]. Taken together, these studies suggest that the expression of ERα/β in the AD brain may play an integral role in the neuroprotective actions of E₂. These effects are discussed in several recent reviews [38,240].

Both ER subtypes ERα and ERβ are implicated in mediating estrogen neuroprotection, although their relative contributions have been incompletely defined. Selective expression of ERα versus ERβ in neural cell lines has suggested a more important role of ERα in mediating neuroprotection in some studies [187] but significant contributions from both ERα and ERβ in others [97,203,218]. Cell culture studies utilizing selective ERα (propylpyrazole triol, PPT), and ERβ agonists (diarylpropionitrile; DPN) to study estrogen neuroprotection typically report similar levels of protection from both agonists, although some evidence suggests greater activity of the ERα agonist PPT [28]. Our studies in primary neuron culture indicate comparable levels of neuroprotection against Aβ from E₂, PPT, and DPN, suggesting potential contributions from both ERα and ERβ in estrogen neuroprotection [67]. However, our data also suggested potential differences between ER subtypes in terms of protective mechanisms, with PPT but not DPN inducing PKC- dependent neuroprotection [67]. Similarly, Brinton and colleagues find that PPT and DPN closely mimic E₂ protection from glutamate excitotoxicity, including activation of ERK signaling and upregula- tion of Bcl-2 expression [387,388]. They also reported differences between the agonists in which DPN effects showed greater calcium dependence [387]. Collectively, available evidence suggests that both ERα and ERβ likely contribute to neuroprotection against AD-related insults but that each may mediate protection by preferentially activate different signaling pathways.

4. Estrogen regulation of β-amyloid accumulation

In addition to increasing neuronal resistance to AD-related insults, estrogen may also protect against AD by preventing the key initiator of AD pathogenesis, accumulation of Aβ. Steady state levels of Aβ are influenced by opposing pathways of Aβ production and Aβ clearance, both of which appear to be regulated by estrogen. Estrogen regulation of Aβ was first suggested by cell culture experiments focused on Aβ production. Early studies demonstrated that estrogen modulates processing of amyloid precursor protein (APP), the transmembrane parent protein of Aβ [106].

The majority of APP is metabolized by two competing pathways, the amyloidogenic and non-amyloidogenic pathways. In the amyloidogenic pathway, thought to occur following endocyto- sis of cell-surface APP, APP is first cleaved by β-secretase (BACE) to liberate β-APPs. The C-terminal fragment (C99/β-CTF) is left embedded in the membrane and is cleaved by the γ-secretase enzyme liberating the Aβ40/Aβ42 peptides. It is thought that another fragment is also released termed the APP intracellular domain (AICD), which can translocate to the nucleus and activate gene transcription. In the non-amyloidogenic pathway, which is the predominant pathway, APP is cleaved within the Aβ domain by α-secretase to liberate a neuroprotective, secreted form of APP (α-APPs). A C-terminal fragment (C83/αCTF) is left embedded in the membrane for further cleavage into non-amyloidogenic fragments [297,343].

Estrogen appears to regulate Aβ levels at least in part by promoting the non-amyloidogenic cleavage of APP, precluding production of the Aβ peptide. In the human kidney 293 cell line, E₂ has been shown to reduce the level of Aβ peptide in a concentration-dependent manner [59]. Further, some preliminary results suggest that in a clinical setting, short-term E₂ treatment is able to reduce plasma levels of Aβ in post-menopausal women naive to HT [20], although the significance of plasma Aβ in terms of both AD pathogenesis and diagnostic value remains controversial.

How estrogen regulates APP processing is not clear, although multiple pathways have been implicated. First, most data suggest that estrogen increases the α-secretase pathway of APP processing [171,213,366,385]. There is evidence that estrogen can promote the α-secretase pathway via activation of extracellular-regulated kinases 1 and 2 (ERK1 and ERK2) signaling [213], a well-established estrogen signaling pathway [319,335,353]. The action of estrogen on the ERK components of mitogen-activated protein kinase (MAPK) signaling pathway and APP generation are rapid and may be ER independent [213]. Estrogen may also regulate APP processing through protein kinase C (PKC)-dependent pathways. PKC signaling is a strong activator of non-amyloidogenic APP processing [74,190,242]. Further, estrogen is a significant activator of PKC in both neuron culture [69,68] and in brain [11,268,299]. Consistent with this possibility, a recent cell culture study has shown that estrogen activation of α-secretase APP processing is blocked by PKC inhibitors [385]. However, not all studies demonstrate such straightforward results. For example, an in vitro study demonstrated that E₂ is capable of increasing the production of sAPPα but not reducing the release of Aβ in cortical neurons over-expressing APP_swe [344].

Some evidence also suggests that estrogen may promote non-amyloidogenic APP processing by altering APP trafficking. Specifically, Greenfield et al. reported that estrogen promoted the secretion of APP containing vesicles from the primary site of amyloidogenic APP processing, the trans golgi nucleus, thereby decreasing available APP substrate for Aβ formation [134]. In addition to promoting the non-amyloidogenic APP processing pathway, estrogen has also been implicated in the modulation of APP levels through the regulation of alternative splicing [333] and APP overexpression postinjury [307], thereby altering substrate for APP processing and subsequent Aβ production.

Importantly, subsequent studies demonstrated that estrogen also functions as a regulator of Aβ in animal models. For example, the depletion of endogenous estrogen by OVX in guinea pigs increased the levels of soluble Aβ in brain, an effect partially reversed by E₂ treatment [258]. This estrogen action may be sex-dependent since androgen but not E₂ treatment reduced elevated Aβ levels resulting from orchiectomy (ORX) of adult male rats [271]. A similar pattern of E₂ regulation of Aβ has been observed in several transgenic mouse models of AD. That is, OVX is associated with increased Aβ and E₂ treatment with reduced Aβ levels in Tg2576 [380,390], APP_swe [199], Tg2576xPS1 [368,390], and 3xTg-AD [53,54] mice. The mechanism by which estrogen regulates Aβ in vivo has yet to be elucidated. In the APP_swe transgenic mouse model of AD, E₂ treatment was associated with increased sAPPα, indicating increased α-secretase APP processing [199]. However, in wild type guinea pigs, experimental manipulation of estrogen status was not associated with corresponding changes in sAPPa levels [258]. Increased levels and activity of BACE observed in aromatase knock-out mice also suggests a potential role for estrogen in the regulation of secretase expression and/or activity [380]. Further work will be needed to elucidate whether estrogen regulation of Aβ in animals involves APP processing and, if so, to define the relevant upstream signaling components (e.g., MAPK, PKC).

Curiously, estrogen levels are associated with Aβ accumulation only in some but not all transgenic mouse models of AD [120,133,148,380]. In the Tg2576, PDAPP, and APP_swexPS1 mouse models, OVX was not associated with increased Aβ levels and E₂ treatment did not reduce Aβ levels. Discrepancies in the effects of estrogen on Aβ levels across transgenic mouse models may reflect several differences, ranging from molecular design of the transgenic lines to variability in methodological parameters such as the timing and dosing of hormone manipulations. One potentially important methodological difference across the studies is their varying techniques for Aβ quantification, each of which preferentially measures different pools of Aβ ranging from soluble monomeric Aβ to oligomeric and deposited forms. Whether estrogen differentially regulates these various Aβ pools is currently unknown. In our laboratory, we found that estrogen decreases Aβ accumulation in the 3xTg-AD mouse model as assessed by the immunoreactive load method [53,54], which detects relatively insoluble intra- and extra-cellular Aβ. Future studies will be needed to determine exactly which Aβ pool(s) estrogen is capable of regulating and whether this contributes to observed differences in estrogen actions across models.

Discrepancies between studies on the role of estrogen as a regulator of Aβ accumulation also may indicate differences in brain levels of estrogen. Recent work suggests that OVX has limitations as a strategy to fully deplete brain estrogens. For example, in the estrogen-responsive element-luciferase mouse model, which was engineered to express the non-mammalian luciferase protein in response to classical ER activation, OVX resulted in relatively high brain estrogen activity in comparison to other body regions [65]. In evaluating the role of brain estrogens in Aβ regulation, Yue et al. [380] found that in the APP₂₃ transgenic mouse model OVX alone was not sufficient to remove all brain estrogen and did not result in elevated Aβ levels. However, they reported elevated Aβ levels after preventing E₂ formation in brain by crossing the APP₂₃ mice with aromatase knock-out mice [380]. Thus, brain levels of sex steroid hormones, which are affected not only by gonadal hormone production but also by de novo steroid hormone synthesis in brain (i.e., neurosteroidogenesis), may be the critical factor in regulation of brain Aβ accumulation. This hypothesized importance of brain hormone levels is supported by the finding that brain levels of E₂ are lower in female AD patients in comparison to age-matched control cases [380], a finding we have recently replicated [287]. In addition, it has recently been shown that long-term OVX in female mice significantly lowers E₂ levels in the hippocampus while increasing serum levels of Aβ [105]. Thus, estrogen regulation of Aβ may depend primarily upon brain estrogen levels, which may be depend on both ovarian and brain steroid production.

In addition to modulating the production of Aβ, estrogen may also promote Aβ clearance. One mechanism of Aβ clearance is through microglial degradation [282]. Estrogen has been shown to promote microglial phagocytosis [47,267] and E₂ treatment increases microglial internalization of Aβ in microglia of both murine [143] and human origin [200]. Estrogen treatment has also been found to reduce Aβ accumulation in rats following intracerebro- ventricular Aβ injection [143]. Correspondingly, increased Aβ burden and impaired microglial Aβ clearance has been reported in an estrogen deficient transgenic mouse model of AD [380]. Estrogen has also been implicated in the regulation of levels of two major Aβ degrading enzymes, insulin degrading enzyme and neprilysin. Both of these enzymes are significant regulators of Aβ levels and their regulation and activities are implicated in AD pathogenesis [331,337]. A few recent studies suggest that estrogen depletion by OVX can decrease neprilysin activity in female rat brain, an effect reversed by E₂ replacement [163]. Thus, estrogens may influence Aβ clearance through regulation of neprilysin, although this pathway may involve an androgen responsive element on the nep- rilysin gene [365]. Estrogen pathways of Aβ-lowering are illustrated in Fig. 1. Given the significance of Aβ accumulation to AD pathogenesis, future studies must clearly define the role of estrogen in regulating both Aβ production and clearance pathways and how they are affected by differences in E₂ brain levels.

5. Aging effects on estrogen responsiveness

Whether the described neuroprotective effects of estrogen prove to have therapeutic relevance to age-related neural diseases including AD will depend in part on the brain’s responsiveness to estrogen with advancing age. One of the primary criticisms of WHIMS and other clinical studies of estrogen-based HT is that the intervention may have been initiated beyond a critical window of opportunity [118,265,276]. This notion refers to the possibility that the aging brain age may lose responsiveness to sex steroid hormones after an extensive period of low hormone levels, such as occurs following menopause. According to this argument, HT may exert estrogenic effects only if begun near the time of menopause. Since most participants in the WHIMS study were many years beyond menopause, the critical window hypothesis could explain in part the absence of beneficial neural actions of HT. Consistent with this position, several clinical studies have noted that HT is associated with positive neural effects in women showing menopause symptoms (e.g., flushing), suggesting retained estrogen responsiveness [154,153,157,306,305]. Although this issue remains to be fully evaluated, results from the Multi-Institutional Research on Alzheimer Genetic Epidemiology study show that HT was associated with reduced AD risk only in post-menopausal women that initiated treatment at a relatively younger age [154].

The critical window notion of an age-related loss in brain responsiveness to estrogen is supported by studies in animal models. One experimental approach to address this issue is the ‘‘gap paradigm” in which OVX animals are treated with E₂ replacement after short versus long periods of time, a design that assesses the effects of prolonged hormone deprivation on subsequent hormone exposure. In general, results from gap studies indicate diminished estrogen responses following many months of hormone absence. For example, E₂ and E₂ plus progesterone replacement was associated with improved spatial memory on the delayed match task when administered within 3 months post-OVX, but not when hormone treatment was delayed 10 months post-OVX [115]. Further, E₂ given immediately after OVX in rats improved spatial memory performance on radial arm maze while E₂ given after 5 months of OVX did not [78]. Recently, it was reported that E₂ given immediately after OVX but not after a 5 months delay was able to increase hippocampal ChAT protein levels in middle-aged OVX wild type rats [35]. Although this topic requires further work to elucidate the key factors and underlying mechanism(s), the data generated thus far suggest that in laboratory animals, the brain can show reduced hormone responsiveness after an extended period of hormone depletion.

Another approach to evaluate the role of aging in neural estrogen responsiveness has been to simply compare the effects of estrogen in young adult versus aging female rodents. This type of study is critical in determining the efficacy of female sex steroid hormones before and after the onset of reproductive senescence, the result of normal reproductive aging in female rodents (reviewed in [58]). While reproductive aging in rodents does not mimic menopause, rodents do experience a pattern of estrus cycle irregularity followed by reproductive senescence that is similar in some respects with the perimenopause period in women [95]. Age-related reproductive changes in female rodents typically become apparent between 9 and 11 months of age, depending upon species and strain. For example, the age-related estrus cycle changes have been well characterized in the C57Bl6 mouse strain [95]. Like women, these female mice demonstrate a large range of variability in cycle irregularity and hormone levels during middle and old age. These mice experience cycle cessation between 11 and 16 mo of age during which a substantial proportion enter a period of persistent vaginal cornification lasting 2-4 months. After this variable period, all mice enter an irreversible final stage of permanent diestrus characterized by low E₂ and progesterone levels and elevated luteinizing hormone levels [114], ovarian follicle depletion, and loss of reproductive capability [126].

In estrogen neuroprotection studies, middle-aged female rodents undergoing reproductive senescence show diminished effects in some studies but retained protection in others. First, several reports suggest that the neural effects of OVX and E₂ treatment are diminished in aging female rodents. Studies by Sohrabji and colleagues suggest that reproductive senescence in middle- aged female rats reduces protective estrogen actions. For example, in assessing estrogen regulation of neurotrophin expression, they found that E₂ treatment in OVX young adult female rats (age 3 mo) increased levels of BDNF, trkA, and trkB in olfactory bulb and diagonal band of Broca, whereas E₂ treatment of middle-aged (17 months) OVX reproductively senescent, female rats showed either no increase or decreased expression of neurotrophins [175]. Similar studies by this research group found that, in comparison to young adult female rats, reproductively senescent female rats show several alterations in estrogen-mediated effects including cytokine and growth factor responses following injury [176,237] and blood-brain-barrier permeability [18]. In another paradigm, Finch and colleagues reported differences between young adult (3 mo) and middle-aged (18 mo) female rats on estrogen regulation of compensatory neuronal sprouting following entorhinal cortex lesion. In comparison to young rats, older rats no longer exhibited an OVX-induced decrease in sprouting and showed differences in regulation of GFAP mRNA [321]. However, some neural actions of estrogen appear to remain relatively robust during aging. For example, E₂ treatment in middle-aged OVX rats enhanced performance on hippocampal-dependent spatial memory tasks [78] as well as altered the hippocampal expression of several genes [1].

In neural injury paradigms, there is evidence of both retained and altered estrogen neuroprotection. In reproductively senescent female rats, both E₂ and progesterone reduced infarct size in a model of stroke injury [5]. Wise and colleagues reported similar neuroprotective effects of E₂ treatment in OVX young (3-4 mo) and middle-aged (9-12 mo) rats in the middle cerebral artery occlusion (MCAO) model of stroke [85,360]. In our laboratory, we have found that reproductively senescent female rats show evidence of altered but not completely diminished estrogen neuroprotection. In young adult (3 mo) female rats, an excitotoxic lesion to hippocampus induced by systemic application of the glutamate agonist kainate is significantly worsened in animals OVX for 2 weeks prior to the lesion. In a similar paradigm, we find that E₂ treatment, initiated at the time of OVX, significantly increases the number of viable hippocampal neurons following kainate lesion [286]. However, in reproductively senescent (14 mo) rats, OVX was not associated with further cell loss, although E₂ replacement still resulted in a significant increase in neuron survival [55]. In a model of spinal cord injury, the effects of E₂ treatment in young (2 mo) versus middle-aged (12 mo) sham-OVX and OVX female rats were investigated. Treatment with E₂ protected against several indices of injury in both young and aging OVX rats, however in ovary-intact rats E₂ neuroprotection was lost in middle- aged rats [60].

Interestingly, emerging data suggest that patterns of reproductive aging in female rats may contribute to altered estrogen responsiveness with age. A recent study compared estrogen protection using the MCAO stroke model in middle-aged female rats stratified by their stage of reproductive aging: reproductively senescent rats that had entered a persistent acyclic state, and rats with normal but lengthened cycles. In comparison to the middle- aged cycling rats, the reproductively senescent rats exhibited larger lesions and no longer showed reduced lesion size following E₂ treatment [298]. Our recent data also suggest that responsiveness to estrogen protection vary according to status of reproductive aging. In the kainate lesion model, we observed that E₂ was most protective in rats showing an acyclic state of persistent vaginal cornification compared to rats with present, albeit irregular cycles [55]. Thus, although additional studies are necessary to define the relationships, it is reasonable to hypothesize that patterns of reproductive aging affect estrogen neuroprotection. Such findings are consistent with the ‘‘critical window” hypothesis of HT and have important implications for the future of clinical use of estrogen-based therapies.

Why the brain shows is less responsive to estrogen with age is unclear, but age-related decreases in ER expression as well as E₂ binding to ERs in aged rat brain have been reported [58,289,361]. Estrogen actions also show age-related changes in other estrogen responsive tissues, including uterus, bone and heart. For example, the uterus becomes less responsive to estrogen with increasing age, showing smaller OVX-induced decreases in uterine weight [367] and uterotrophic effects of estrogen only when treated soon after OVX [78]. Further, while most studies demonstrate that the trophic effects of E₂ on bone extend through middle-age, some studies suggest that as aging progresses, this effect is altered from an ERα/β-mediated effect to only an ERα mediated one [168]. Interestingly, increased ERα predominance has also been suggested to underlie age changes in neural estrogen responsiveness [19].

Taken together, available experimental research suggests that although estrogen neuroprotection is often observed in aging rats, it can also be significantly diminished. Still uncertain is how the many AD-related facets of estrogen neuroprotection may be impacted by aging. Additional studies are necessary to determine the extent to which observed age-related changes in estrogen responsiveness may be delayed, prevented and or reversed. This phenomenon has important implications for the future of HT in post-menopausal women. Ongoing clinical and animal studies promise to shed new insight into this issue in the next few years

6. Progesterone interactions with estrogen in regulation of Alzheimer’s disease

Estrogen actions must also be considered with respect to the second major class of ovarian hormones, progestogens. Progesterone has long been recognized as a regulator of estrogen, particularly in the female reproductive system (reviewed in [130]), where it often antagonizes estrogen action. Clinically, progesto- gens are typically a key element of HT that are thought to minimize deleterious effects of estrogen. Perhaps most importantly, in experimental paradigms progesterone can inhibit human endometrial cancer cell growth [77,79] and in clinical studies progestogens are associated with reduced risk of endometrial cancer [129,257].

In clinical studies of AD and dementia, most results indicate similar outcomes with both estrogens alone (ie., CEE) and estrogens in combination with progestogens (ie., CEE + MPA). In the WHIMS trial, a comparison between the CEE alone and CEE + MPA arms of the study raised the question that the clinical efficacy of HT may be dependent upon the hormone constituents within. Both the CEE and CEE + MPA arms failed to demonstrate a protective effect and both actually increased the risk of dementia compared to women receiving placebo [311]. Notably, the CEE alone arm had a negative impact on global cognitive function, however this negative impact was worsened when pooled with the data from the CEE + MPA arm [91]. Interestingly, short-term HT treatment in women with existing AD was associated with some benefits on psychiatric symptoms in the CEE group but not in the CEE + MPA group [162]. These and related issues suggest that the inclusion of a progestogens may influence the effects of CEE alone on cognitive outcomes and risk of dementia in post-menopausal women [75].

Despite the common use of the progestogen MPA in current HT paradigms, researchers have only relatively recently begun to investigate the effects of progesterone on the CNS in regards to aging and neurodegenerative diseases. Although compelling experimental evidence indicates numerous protective actions of estrogen and progesterone when delivered independently [44,295], comparatively less is known about interactions between estrogen and progesterone when they are administered together. Interestingly, accumulating observations indicate that progesterone often antagonizes rather than synergizes with estrogen-mediated neuroprotective actions. This incomplete understanding highlights the need to determine the interactive effects of these hormones in the brain.

Findings from an increasing body of research have begun to provide insight into how beneficial neural actions of estrogen are affected by interactions with progesterone. Findings from both in vitro and in vivo paradigms suggest that progesterone treatment can antagonize estrogen neuroprotection. For example, long-term progesterone treatment in aged (23-24 mo) OVX female rats blocked estrogen upregulation of brain derived neurotrophic factor, nerve growth factor, and neurotrophin 3 [32]. In a similar paradigm of hormone treatment in middle-aged OVX rats, estrogen-induced improvement in spatial memory performance was blocked by co-administration of progesterone [33]. More specific to neuron viability though are recent studies from our laboratory demonstrating that progesterone blocks estrogen neuroprotection from excitotoxic injury in female rats. In both young adult (3 mo) [286] and middle-aged (14 mo) [55] OVX rats, continuous E₂ treatment reduced kainate-induced neuron loss in hippocampus CA2/3 whereas continuous progesterone did not significantly affect neuron viability. Importantly, when progesterone was included with E₂, estrogen neuroprotection was no longer observed [55,286]. Similarly, Brinton and colleagues recently reported that E₂ and progesterone administered independently to wild type rats enhanced several markers of brain mitochondrial function but, when replaced in combination, the hormones showed attenuated rather than enhanced responses [167].

While the mechanisms behind this antagonistic property remain unknown, our laboratory has begun to investigate the possibility that progesterone modulates estrogen function in part by regulating ER expression. We have observed in primary neuron culture that low physiological concentrations of progesterone rapidily downregulate mRNA levels of both ERα and ERβ [174]. Functionally, this progesterone-mediated decrease in ER expression was associated with inhibition of both ERE-mediated transcriptional activity and estrogen neuroprotection against apoptosis [174]. Although progesterone treatment by itself did not significantly affect apoptosis in this paradigm, abundant evidence demonstrates that progesterone can activate several neuroprotective cell signaling pathways, including Akt [318] and ERK [233,232] signaling and upregulation of the anti-apoptotic protein Bcl-2 [232]. Further, as reviewed in an accompanying article, progesterone can significantly protect neurons against numerous insults [44,295]. Thus, independently estrogen and progesterone can exert protective actions, but in combination they can inhibit each other and thus fail to protect. Perhaps not unexpectedly, the interactive neuroprotective effects of the two female sex steroid hormones are not quite so straightforward. Besides antagonistic effects, progesterone can also synergistically interact with estrogen to promote beneficial neural effects including increased spine density [100,313]. In OVX female rats, acute progesterone treatment (2-6 h) was observed to augment the estrogen-induced increase in spine density, whereas prolonged progesterone treatment (18 h) blocked the estrogen effect [362]. Thus, a key factor in understanding estrogen neuroprotection and perhaps its relevance to HT in post-menopausal women is elucidation of the interactions between estrogen and progesterone.

A similar progesterone regulatory relationship also appears to affect estrogen protection from indices of AD-like neuropathology. In recent studies, our laboratory has begun to investigate how progesterone interacts with estrogen in regulating Aβ accumulation in the 3xTg-AD transgenic mouse model of AD. As discussed above, we found that 3 months following OVX of young adult female 3xTg-AD mice, there were robust increases in Aβ accumulation and tau phosphorylation and impaired performance in spontaneous alternation behavior in comparison to sham OVX 3xTg-AD mice [54]. Continuous E₂ treatment during the 3 month OVX period largely prevented the worsening of AD-like neuropathology and behavioral performance, however continuous progesterone by itself did not affect OVX-induced changes in either Aβ accumulation or behavior. Consistent with an antagonistic role of progesterone, we observed that co-treatment with progesterone antagonized the beneficial effect of estrogen in lowering Aβ accumulation [54]. However, estrogen and progesterone co-treatment did show a beneficial effect in reducing levels of tau hyperphosphorylation, suggesting positive estrogen-progesterone interactions. Consistent with a beneficial role of progesterone, Frye and colleagues report that long-term progesterone treatment following OVX in another transgenic mouse model of AD was associated with some cognitive benefits [104]. Taken together, these studies provide the first information to date on the interactive effects of estrogen and progesterone on AD-like neuropathology and demonstrated the potential for both positive and negative outcomes in terms of protection from AD-related neuropathology.

Despite evidence of antagonistic neural effects of progesterone on estrogen neuroprotective actions, progestogens are still deemed a necessary component of HT in women with a uterus. Therefore, HT may need to be optimized to maximize the benefits and minimize the unwanted consequences associated with estrogen-progestogen interactions. One possible strategy is the use of cyclic hormone delivery rather than the continuous, combined treatment that is currently common to HT. Initial clinical evaluation of cyclic progestogen exposure has been completed and more is underway. Several clinical HT trials have incorporated a comparison between continuous versus cyclic progestogen in post-menopausal women on osteoporosis and cognitive function. For example, two completed studies have both demonstrated that long-term HT with a cyclic progestogen dose was able to increase bone mineral density in post-menopausal women [239,56]. Similarly, a continuous estrogen plus cyclic progestogen paradigm is currently employed in the KEEPS (Kronos Early Estrogen Prevention Study) Cognitive and Affective Study, a randomized, placebo-controlled, doubleblind study investigating the effects of HT in post-menopausal women who are within 36 months of their final menstrual period [357]. This and similar new trials promise to provide important insight on the efficacy of cyclic hormone delivery and the hypothesized importance of a critical window of hormone intervention.

Experimental studies in animal models lend support for the use of cyclic rather than continuous progesterone to optimize estrogen neuroprotection. For example, Gibbs have demonstrated that short-term treatment with E₂ and progesterone can improve cholinergic function [116], with maximal benefit resulting from cyclic administration of estradiol and progesterone and the least benefit from continuous combined treatment [115]. In our laboratory, we have begun investigating the potential utility of cyclic progesterone against AD neuropathology by comparing cyclic versus continuous progesterone in the presence and absence of continuous E₂ in OVX 3xTg-AD mice. Our results show that whereas continuous progesterone largely inhibits estrogen protection from AD-related neuropathology, cyclic progesterone appears to significantly increase estrogen protection against Ap accumulation, tau phosphorylation, and working memory deficits (unpublished observations). These exciting new findings support the hypothesis that estrogen- progesterone interactions can yield additive neuroprotection and that cyclic hormone delivery may be a critical parameter.

7. Age-related androgen depletion and Alzheimer’s disease

In parallel to the relationships between age-related estrogen loss in women and increased AD risk, testosterone is depleted as a normal consequence of aging in men and is linked with elevated risk of AD. As discussed above, a significant biological event in women that contributes to the role of aging in AD is menopause and the resultant loss of the sex steroid hormones estrogen and progesterone. Although men do not experience menopause per se (i.e., a cessation of reproductive ability, nearly complete loss of sex steroid hormones), men do experience a somewhat similar process termed androgen deficiency in aging males (ADAM). ADAM refers to normal, age-related depletion of testosterone and the corresponding constellation of symptoms that reflect dysfunction and vulnerability to disease in androgen-responsive tissues including brain [24,50,96,125,179,183,217,224,302]. The decline in testosterone levels begins in the 3rd decade and continues at an annual rate of 0.2-1% for total testosterone and 2-3% for bioavailable testosterone [94,131,227]. Unlike menopause, aging men do not experience comparable levels of andropause. That is, although all men exhibit significant age-related testosterone loss typically beginning in the third decade of life, men vary in the extent of testosterone loss and the corresponding severity of clinical manifestations [226,326]. It is estimated that 30-70% of men aged 70 and older are hypogonad- al, resulting in at least 5 million aging men in the US suffering the consequences of andropause and only a small minority of those receive hormone treatment [142,225]. ADAM is associated with increased risk of sarcopenia, osteoporosis, falls, frailty, and all cause mortality.

The brain is a highly androgen responsive tissue where androgens induce several beneficial actions. For example, androgens have been shown to improve mood and promote select aspects of cognition, including spatial abilities [127,172] and verbal fluency [4]. Men with a higher free testosterone index have been found to perform better on visual and verbal memory and exhibited better long-term memory [23], while those with a low free testosterone index can show decreased visual memory, visuomotor scanning, verbal memory, and visuospatial processing [219]. ADAM has been associated with impaired cognitive performance in some but not all studies [141,219].

One recently established consequence of ADAM is an increased risk for the development of AD. Several [161,160,159,274,352] but not all [255] studies have identified a relationship between low circulating levels of testosterone and a clinical diagnosis of AD. In these studies, the relationship between testosterone and AD appears to be strongest when circulating levels of free rather than total testosterone are examined, and when mean ages are under 80 years [161,160,159,249]. The relationship between testosterone and AD may be influenced by the presence of at least one apolipo- protein ε4 allele, a genetic risk factor for AD [322]. Specifically, men with at least one ε4 allele had lower levels of testosterone than men without an ε4 allele [160]. Animal studies support a link between apolipoprotein E, testosterone, and AD [269,270].

Although the majority of studies have identified a relationship between low testosterone and increased AD risk in men, most were unable to determine whether low testosterone contributes to the disease process or is merely a result of it. However, two complementary studies suggest low testosterone occurs prior to or in the early stages of AD pathogenesis, and thus likely acts a risk factor. The first study compared clinical diagnosis of dementia with blood levels of testosterone in the prospective Baltimore Longitudinal Study on Aging [220]. Male subjects were followed for 4-37 years with a mean of 19 years per subject and were diagnosed as clinically normal at the time of their first testosterone measurement. Subjects that eventually received a clinical diagnosis of AD showed lower circulating levels of free testosterone. Interestingly, in those men with AD, testosterone levels were reduced at check-ups 5-10 years prior to diagnosis [220], suggesting androgen loss occurred well before clinical manifestations of the disease. Consistent with this study are findings from a study by our laboratory in which we linked low brain levels of testosterone with increased risk of AD in men [284]. First, using human postmortem brain tissue from neuropathologically normal men, we found that levels of testosterone but not E₂ show a significant age-related decline. When we examined changes in brain levels of hormones across cases stratified by neuropathological status, we found significantly decreased brain levels of testosterone in AD cases as compared with neuropathologically normal cases even after controlling for the age-related hormone loss [284]. We also measured brain levels of androgens in cases with mild neuropathological changes, consistent with the earliest stages of AD. These cases also exhibited low testosterone levels [284], again indicating that testosterone loss occurs prior to robust pathology and thus may contribute to the development of AD. Taken together, these results suggest age-related testosterone depletion in men is a risk factor for AD.

Unlike the numerous clinical studies that have evaluated the efficacy of HT use in treating and or preventing AD in post-menopausal women, comparatively few studies have examined testos terone therapy in men for protective roles against age-related cognitive decline and development of dementia. Androgen therapies have been approved and used for the treatment of hypogonadism in men and are typically associated with reduced fat mass and improved muscle mass and bone density as well as increased mood, libido, and overall quality of life [31,327,332]. However, clinical evaluation of beneficial cognitive effects of testosterone therapy have been mixed. For example, a study of hypogonadal and eugonadal men found that testosterone increased verbal fluency [4]. Others report improvements in spatial cognition and working memory following testosterone treatment [62,173,172]. In contrast, some studies did not find significant changes in cognition following testosterone therapy [30,90,141,210,339].

A few studies have evaluated the effects of testosterone therapy in subjects with AD. In a small clinical study of men recently diagnosed with AD, testosterone treatment for up to 1 year contributed to improvement in both overall cognitive ability and visual spatial skills [328]. In contrast, other studies have not reported significant benefits of testosterone therapy in men with mild cognitive impairment and AD [63,206]. As with the observed inconsistencies in the literature of HT use in women, there are likely several factors that contribute to the observed differences between testosterone studies, including cognitive domains, treatment type and duration, and the age and other characteristics of the subjects.

8. Testosterone neuroprotection and Alzheimer’s disease insults

One beneficial action of androgens that is hypothesized to contribute to a role in reducing risk of AD is neuroprotection. Androgens are established promoters of neuron viability during neural development as well as in adult brain following mechanical injury and disease-related toxicity. One target of androgen neuroprotection is motorneurons following axotomy [178]. In this paradigm, testosterone treatment accelerates the rate of nerve regeneration and attenuates neuron loss [164,178,177,193,194,195,330, 377,379]. Similarly, following facial nerve crush in male hamsters, testosterone increased the rate of axonal growth and functional recovery [193,192]. These effects are true not only for testosterone but also its potent androgen metabolite dihydrotestosterone (DHT) [378,377,379]. In addition, the anti-androgen flutamide was able to block testosterone’s neuroprotective effects on motor neurons [195], corroborating the role of androgen versus estrogen pathways. The mechanism behind this neuroprotective action appears to be through androgen regulation of trophic factors [378,379]. Recently, studies have found that in addition to long-term treatment, short-term testosterone, DHT, and estrogen treatments are protective and suggest a more direct mechanism of hormone action [164].

In addition to neuroprotective effects on motor neurons, androgens have also been found to promote neuron survival in brain regions vulnerable to neurodegenerative diseases such as Alzheimer’s disease. These areas include the hippocampus and cortical regions, which are both affected in AD and rich in androgen receptors [314]. In a study by Garcia Segura and colleagues, acute testosterone treatment attenuated neuron loss in the hilus of the dentate gyrus following excitotoxic lesion in ORX male mice [16]. Interestingly, acute treatment of E₂ was also protective while DHT treatment did not protect against neuron loss in androgen depleted mice. Furthermore, the protective effect of testosterone was blocked by an aromatase inhibitor, suggesting that in this model of acute hormone treatment, estrogen is responsible for testosterone neuroprotection [16]. A study from our laboratory investigated the effect of long-term hormone replacement on neuronal death induced by excitotoxic lesion. In this study, depletion of endogenous androgens as a consequence of ORX resulted in increased hippo campal neuron loss following kainate lesion in comparison with sham ORX rats. However, in ORX rats treated for two weeks with DHT, this increase in cell loss was blocked [272]. In contrast to the results of Garcia Segura and colleagues, we found that rats treated with E₂ did not exhibit decreases cell loss following kainate lesion. This suggests that androgen regulation of neuroprotection in this paradigm is a result of androgen rather than estrogen pathways. Behavioral responses to seizures were measured and no differences were observed between groups suggesting that the observed androgen neuroprotection was not a result of decreased seizure severity [272]. Although we did not observe an androgenmediated effect on seizure severity, other studies have found a significant effect of androgens on seizure severity. In studies by Frye and colleagues, testosterone decreased neuron loss by inhibiting seizure activity [102,103]. Subsequent studies found that protection was due to DHT metabolism to 5α-androstane-3α, 17α-diol, an androgen that acts on GABAA receptors. GABA activation reduces excitatory signaling, which in turn attenuates seizure activity thereby minimizing lesion severity [87,88,277].

While in vivo models provide valuable insight into androgen neuroprotection, neuron culture models of toxicity have proven valuable in defining the underlying molecular mechanisms. Cell culture models of neural injury have demonstrated testosterone protection against serum deprivation [45,138], Ap toxicity [229,260,384], and oxidative damage [2]. Testosterone neuroprotection against serum deprivation-induced apoptosis requires activation of an androgen receptor (AR) dependent mechanism [138]. Specifically, the anti-androgen flutamide attenuated protection while an aromatase inhibitor had no effect on neuron viability [138]. Consistent with this androgen-mediated mechanism of androgen neuroprotection is an early study from our laboratory, which found that testosterone neuroprotection against toxicity induced by extracellular Ap results from DHT not E₂ [260]. DHT treatment in this paradigm was equally as protective as testosterone, but use of an anti-estrogen droloxifene failed to block protection, suggesting androgen pathways are responsible for neuroprotection [260]. Further, we observe androgen neuroprotection in PC12 cells transfected with AR but not in either untransfected PC12 cells or those transfected with empty vector [229].

There are several potential mediators of androgen neuroprotection downstream of AR. Some evidence suggests androgen neuroprotection may be mediated through attenuation of oxidative stress [2]. Another potential mechanism involves a classic genomic mechanism, increased expression heat shock proteins. Androgen attenuation of Ap induced toxicity was associated with elevated levels of heat shock protein 70, which is known to participate in protective responses against cellular stress and neurodegeneration [384]. Recent work from our lab identified a non-genomic, AR- dependent mechanism involving activation of a mitogen-activated protein kinase (MAPK)/extracellular signal regulated kinase (ERK) pathway [229]. We observed that physiological levels of testosterone and DHT rapidly and transiently activated MAPK/ERK signaling in cultured hippocampal neurons. Downstream of ERK, we found that androgens activated p90 kDa ribosomal S6 kinase (Rsk), which in turn phosphorylates the pro-apoptotic protein Bad. Phosphorylation of Bad results in its inactivation of Bad, thereby tilting the balance of apoptosis towards increased cell viability. Pharmacological inhibition at any step of this pathway prevents both phosphorylation of Bad and androgen neuroprotection [229]. Confirming the non-genomic nature of this pathway, we found that in this neuron culture paradigm the classic anti-androgens flutamide and cypro- terone acetate mimicked neuroprotection against apoptotic insults afforded by testosterone and DHT even though they also blocked classic genomic actions of androgens [231]. Further, protective actions of flutamide and cyproterone acetate were only observed in AR-containing cell lines. Whether activation of protective androgen pathways requires membrane AR, intracellular AR, or both is unclear. We observed that testosterone conjugated to albumin - which is designed to prevent cell entry and thus permit only activation of membrane receptors - was ineffective in activating protective MAPK/ERK androgen signaling [229]. Similarly, Gatson and colleagues reported that while DHT phosphorylates both ERK and Akt, albumin-conjugated DHT resulted in dose-dependent suppression of ERK signaling in glioma cells expressing AR [113]. Pathways of androgen neuroprotection are summarized in Fig. 2.

Figure 2. Androgens activate neuroprotective pathways that may attenuate Alzheimer’s disease. First, testosterone (T) is aromatized in brain to 17β-estradiol (E₂), which activates estrogen-mediated neuroprotective pathways (summarized in Fig. 1). Second, testosterone and its metabolite dihydrotestosterone (DHT) activate AR-dependent protective pathways. T and DHT reduce neuronal apoptosis by a non-genomic signaling cascade involving activation of MAPK/ERK, followed by activating phosphorylation (p) of Rsk, and inactivating phosphorylation of the pro-apoptotic protein Bad. Also, androgens decrease levels of the AD-related protein Ab by a classic genomic mechanism involving activated AR interaction with androgen response elements (ARE) on the neprilysin gene, which results in increased expression of this Ab-catabolizing enzyme.

In contrast to these observations of androgen neuroprotection, there appear to be circumstances in which androgens fail to protect against neural injury and can even exacerbate insults. For example, Dluzen and colleagues found that E₂ but not testosterone reduces methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) nigrostriatal dopaminergic toxicity [82]. Further, testosterone has been found to exacerbate neuron loss following middle cerebral artery occlusion (MCAO) [372,371]. In this model removal of testosterone 6 h prior to MCAO reduces lesion volume by as much as half [372,371]. It is not clear whether the absence of androgen neuroprotection in these paradigms reflects roles of brain region, insult, and or other factors. One important issue in at least some studies may be androgen concentration. That is, at physiological low nanomolar levels testosterone can protect against excitoxicity in cultured neurons, but worsens cell death when present at supraphysiological micromolar levels [244]. Similarly, micromolar but not nanomolar concentrations of testosterone were associated with increased calcium signaling and neuronal apoptosis [92,112]. Thus, although there is ample evidence of androgen neuroprotection against AD-related insults, androgen effects on neuron viability may depend on a number of factors, including brain region, type of insult, and hormone concentration.

In addition to direct neuroprotection, androgens also protect against another form of neuropathology directly relevant to AD, hyperphosphorylation of tau. Abnormal, excessive phosphorylation of the cytoskeletal protein tau in the form of neuropil threads and neurofibrillary tangles is a defining neuropathological characteristic of AD and several other neurodegenerative disorders [166,355,358]. Although relatively little research has examined the relationship between androgens and tau hyperphosphorylation, available evidence does indicate that androgens are protective against this pathology. Papasozomenos and colleagues have examined the effects of testosterone and E₂ on tau hyperphosphorylation using a model of heat shock-induced phosphorylation. In this paradigm, testosterone but not E₂ prevents tau hyperphosphorylation [252,250,251]. In addition to affecting phosphorylation of tau, recent evidence suggests that androgens also regulate tau cleavage [253]. Specifically, testosterone prevented calpain-medi- ated tau cleavage preventing the generation of the 17-kDa tau fragment [253].

9. Testosterone regulation of β-amyloid accumulation

In addition to classic neuroprotective actions, androgens may also protect the brain from AD by regulating accumulation of Aβ. Initial work suggesting a relationship between androgens and Aβ came from a small study evaluating men treated with anti-androgen therapies for prostate cancer. Gandy and colleagues found that within several weeks following initiation of anti-androgen therapy (consisting of leuprolide and flutamide), circulating levels of testosterone and E₂ were largely depleted whereas plasma levels of Aβ were significantly elevated [107]. Martins and colleagues similarly reported an association between androgen depletion and elevated Aβ levels in males receiving anti-androgen therapy for the treatment of prostate cancer [6] and in older males suffering from memory loss or dementia [117]. To what extent these observations reflect effects of androgen versus estrogen pathways or perhaps refleet associated changes in gonadotropins remains incompletely resolved [13,283]

Consistent with the observations in aging men, experimental work in rodents also indicates that androgens function as endogenous negative regulators of Aβ. Early studies in our laboratory demonstrated that androgens but not estrogens reduce brain levels of soluble Aβ in male rats [271]. Specifically, we found that ORX-in-duced depletion of endogenous androgens was associated with a modest but significant increase in soluble Aβ from hemi-brain lysates. Further, DHT treatment in ORX rats resulted in a significant reduction in brain Aβ levels, although E₂ treatment had no effect [271]. We observed similar findings on the relationship between androgen levels and Aβ accumulation in the 3xTg-AD mouse model of AD. Male 3xTg-AD mice were depleted of androgens by ORX at age 3 mo, a time prior to the development of significant AD-like pathology [238]. Mice were exposed immediately to either DHT or vehicle, treatments that were maintained continuously for the next 4 months. After the treatment period, we observed significant intracellular accumulation of presumably insoluble Aβ in gonadally intact, sham ORX 3xTg-AD mice (age 7 mo) in the CA1 hippocampus, subiculum, and amygdala [285]. In comparison to the sham ORX animals, the ORX mice showed significantly higher levels of Aβ in all three brain regions as well as significantly poorer behavioral performance on a spontaneous alternation task [285]. The elevated pathology and exacerbated behavioral impairments in the ORX group were blocked in ORX mice treated with DHT, suggesting a preventive effect of androgens in regulation of AD-related pathology. Confirming our findings in animal models, a recent study reported that levels of Ap in plasma and cerebrospinal fluid were significantly elevated following ORX in guinea pigs, an effect prevented by testosterone replacement 1 week following ORX [346].

There are likely several mechanisms that contribute to androgen regulation of Aβ (Fig. 2). One obvious possibility is that aromatase-mediated conversion of testosterone to E₂ in brain allows activation of the several estrogen pathways of Aβ regulation discussed above. In fact, cell culture studies are consistent with indirect testosterone activation of estrogen-mediated regulation of APP processing. The first study to examine testosterone regulation of APP and Aβ in culture found that prolonged testosterone treatment was associated with increased α-secretase cleavage of APP and reduced Ap [128]. Unclear was whether these findings involved direct androgen pathways, indirect activation of estrogen pathways, or both. In a subsequent culture study, testosterone was also observed to promote proteolysis of APP by α-secretase, however this effect was blocked in the presence of aromatase inhibitors suggesting estrogen dependence [121]. In our study of male rats in which androgens were found to regulate brain levels of soluble Ap, we did not observe detectable differences in either full-length APP or APPa across androgen treatment groups [271], perhaps indicating that alterations in APP processing are not the only mechanism by which androgens affect Ap levels.

In addition to regulating Aβ generation via effects on APP proteolysis, androgens may also decrease Ap levels by promoting endogenous clearance pathways. Recent evidence from our group demonstrates that androgens reduce Ap levels as a consequence of regulating expression of the Aβ-catabolizing enzyme neprilysin [376], a critical enzyme in homeostasis of brain Ap [170]. In neural cell cultures, we found that androgens robustly increase protein levels of neprilysin. A classic, AR-dependent genomic mechanism is implicated since (i) the neprilysin gene contains androgen response elements [304], (ii) neprilysin regulation was blocked by the anti-androgens flutamide and cyproterone acetate, and (iii) androgens only increased neprilysin in AR-containing cells [376]. Over-expression of human APP in cultured cells resulted in elevated levels of soluble Ap that were reduced by testosterone and DHT. Further, we found that pharmacological inhibition of either AR or neprilysin blocked the ability of androgens to reduce Aβ levels, suggesting that the Aβ-lowering actions of androgens is mediated by AR-dependent regulation of neprilysin expression [376]. Importantly, these observations were replicated in male rats. We found that ORX-induced androgen depletion resulted in decreased levels of NEP and elevated Aβ, and DHT replacement restored NEP and Aβ to levels observed in sham GDX animals [376].

10. Emerging strategies of hormone-related therapies in Alzheimer’s disease.

As reviewed in this article, there are numerous neuroprotective actions of estrogens and androgens that have direct relevance to AD pathogenesis and compelling potential to prevent and possibly treat the disease. However, the promise of estrogen-based and androgen based HTs in reducing AD risk have yet to be realized. As findings and directions from clinical and basic science research become increasingly integrated, it is anticipated that critical parameters affecting HT efficacy will be optimized, including age of HT initiation as well as the formulation, regimen, and delivery of HT. As ongoing research continues to address these crucial and immediate concerns, an emerging area of investigation is the development of natural and synthetic hormone mimetics that will preferentially activate estrogen and androgen neuroprotective mechanisms while minimizing deleterious consequences in other tissues.

Estrogen compounds that show tissue-selective agonist actions are termed selective estrogen receptor modulators (SERMs). Currently the most studied and clinically relevant SERMs are tamoxifen and raloxifene, synthetic compounds that exhibit tissue-dependent ER agonist and antagonist actions. As a potent antagonist of estrogen action in breast tissue, tamoxifen is best recognized as an antiestrogen used to treat breast cancer although it can exert agonist ER effects on bone and lipids [48,300]. Interestingly, low concentrations of tamoxifen can protect cultured neurons from toxicity due to Ap and glutamate [243], suggesting the potential for a protective role against AD. However, tamoxifen has also been observed to block E2-mediated protection in cultured neurons [57,383]. Potential benefits of tamoxifen use in post-menopausal women for prevention or treatment of AD has not been well studied. Some studies indicate increased risk of cognitive deficits in tamoxifen users [246,308], whereas another study suggested tamoxifen may reduce AD risk [41]. Like tamoxifen, raloxifene antagonizes estrogen actions in breast but has agonist actions on bone [48,81]. In brain, raloxifene mimics some but not all protective estrogen actions. Raloxifene increases choline acetyl- transferase in hippocampus of OVX rats [363] and in cultured neurons it increases neurite outgrowth [235] and can reduce Ap toxicity [243]. Conversely, E2 but not raloxifene was effective in attenuating Ap-induced inflammatory reaction in OVX rats [334]. In post-menopausal women, raloxifene use has been linked with reduced risk of cognitive impairment and development of AD [370].

Currently, effort is being focused on next generation SERMs that exhibit more robust and specific neuroprotective actions [42,303]. For example, Brinton and colleagues recently developed a synthetic SERM with both estrogenic and antioxidant potential that protects cultured neurons from cell death [389]. Evaluation of such compounds in animal models of AD is only beginning. Towards this end, our laboratory has begun to investigate two particular SERMs, propylpyrazole triol (PPT) and diarylpropionitrile (DPN), which show relative specificity for ERα and ERβ, respectively. Although we observe that both compounds protect cultured neurons from Aβ toxicity [67], PPT but not DPN treatments effectively mimicked E2 in reducing Aβ accumulation and improving behavioral deficits in OVX 3xTg-AD mice [53].

In parallel to the ongoing research on SERMs, there has been a recent growth in the interest and development of tissue-specific selective androgen receptor modulators (SARMs) [108,147,186]. Given the prevalence of ADAM and its widespread deleterious consequences, androgen based therapy is of considerable interest. However, prostate cancer, which is the second leading cancer among aging men in terms of both prevalence and cause of death [99], is androgen-dependent and typically treated by androgen deprivation therapy. Consequently, the use of testosterone therapy with its potential to increase risk and or progression of prostate tumorigenesis has been controversial [183] and promoted the development of SARMs that lack significant androgen action in prostate but exert agonist effects in select androgen-responsive tissues of interest, including brain, muscle, and bone.

Several strategies of SARM design are currently been pursued [46,108,359]. One strategy is to develop novel steroidal compounds that are not substrates for the enzyme 5a-reductase or yield reduced metabolites with minimal androgencity. Testosterone is converted to DHT by the actions of 5a-reductase, an enzyme localized in specific target tissues such as prostate. Prostate growth depends largely on the actions of DHT rather than T because DHT exhibits ~10-fold greater net potency, which reflects both a higher binding affinity for AR and a slower dissociation rate from AR [359]. SARMs that are not 5a-reductase substrates or form DHT- like derivatives with weak androgenic activity have low androgen action in prostate [108,188,201,211,245,301,342]. A promising SARM in this category is 7α-methyl-19-nortestosterone, commonly called MENT [223,301,342], which was developed by the Population Council and is currently in clinical trials as an androgen therapy for hypogonadal men [10,345]. MENT shows low androgen activity in prostate but is more potent than T in other peripheral androgen-responsive tissues including bone [76,301,342] and muscle [76,301,342]. The effects of MENT on neural function are virtually unknown, but it has been shown to mimic the ability of testosterone to induce sexual behavior in castrated rats [223] and is a robust regulator of the hypothalamic-pituitary-gonadal axis, suggesting neural efficacy.

Another SARM design strategy is the development of non-steroidal synthetic AR ligands. One such SARM is flutamide, which mimics the abilities of T and DHT to increase hippocampal spine density in both male and female rats [207,208]. In cultured neurons, we have found that flutamide antagonizes classic genomic actions of T and DHT but mimics rather than blocks the nongenomic neuroprotective actions of these androgens [230]. Of particular interest are novel compounds that bind AR but have altered interactions with AR binding pocket side chains that underlie tissue specificity. Recent research has determined that although synthetic SARMs must closely mimic the rigid backbone core structure of the AR ligand binding domain, there can be extensive variation in how they interact with amino side chains in the binding pocket [359]. Thus, SARMs are predicted to exert tissue-specific effects dependent in part upon how they interact with amino acid sidechains in the AR binding pocket [36,46,201]. Recent research indicates success in developing SARMs with desired tissue specificity [245]. As with SERMs, the development of brain-specific SARMs that exert neuroprotective actions associated with reduction of AD pathogenesis is a key topic of ongoing research.

Acknowledgements

This work was supported by NIH Grants AG026572 and AG23739. JCC was supported by NIH Grant F31NS059174.

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References

 K.K. Aenlle, A. Kumar, L. Cui, T.C. Jackson, T.C. Foster, Estrogen effects on cognition and hippocampal transcription in middle-aged mice, Neurobiol. Aging (2007).

 E. Ahlbom, G.S. Prins, S. Ceccatelli, Testosterone protects cerebellar granule cells from oxidative stress-induced cell death through a receptor mediated mechanism, Brain Res. 892 (2001) 255-262.

 H. Akiyama, S. Barger, S. Barnum, B. Bradt, J. Bauer, G.M. Cole, N.R. Cooper, P. Eikelenboom, M. Emmerling, B.L. Fiebich, Inflammation and Alzheimer's disease, Neurobiol. Aging 21 (2000) 383-421.

 G.M. Alexander, R.S. Swerdloff, C. Wang, T. Davidson, V. McDonald, B. Steiner, M. Hines, Androgen-behavior correlations in hypogonadal men and eugonadal men. II. Cognitive abilities, Horm. Behav. 33 (1998) 85-94.

 N.J. Alkayed, S.J. Murphy, R.J. Traystman, P.D. Hurn, V.M. Miller, Neuroprotective effects of female gonadal steroids in reproductively senescent female rats, Stroke 31 (2000) 161-168.

 O.P. Almeida, L. Flicker, Testosterone and dementia: too much ado about too little data, J. Br. Menopause Soc. 9 (2003) 107-110.

 O.P. Almeida, N.T. Lautenschlager, S. Vasikaran, P. Leedman, A. Gelavis, L. Flicker, A 20-week randomized controlled trial of estradiol replacement therapy for women aged 70 years and older: effect on mood, cognition and quality of life, Neurobiol. Aging 27 (2006) 141-149.

 M. Alvarez-De-La-Rosa, I. Silva, J. Nilsen, M.M. Perez, L.M. Garcia-Segura, J. Avila, F. Naftolin, Estradiol prevents neural Tau hyperphosphorylation characteristic of Alzheimer's disease, Ann. NY Acad. Sci. 1052 (2005) 210-224.

 K. Andersen, L.J. Launer, M.E. Dewey, L. Letenneur, A. Ott, J.R.M. Copeland, J.-F. Dartigues, P. Kragh-Sorensen, M. Baldereschi, C. Brayne, A. Lobo, J.M. Martinez-Lage, T. Stijnen, A. Hofman, Gender differences in the incidence of AD and vascular dementia: the EURODEM studies, Neurology 53 (1999) 1992-1997.

 R.A. Anderson, C.W. Martin, A.W. Kung, D. Everington, T.C. Pun, K.C. Tan, J. Bancroft, K. Sundaram, A.J. Moo-Young, D.T. Baird, 7a-Methyl-19- nortestosterone maintains sexual behavior and mood in hypogonadal men, J. Clin. Endocrinol. Metab. 84 (1999) 3556-3562.

 M.A. Ansonoff, A.M. Etgen, Estradiol elevates protein kinase C catalytic activity in the preoptic area of female rats, Endocrinology 139 (1998) 30503056.

 B. Antonsson, J.C. Martinou, The Bcl-2 protein family, Exp. Cell. Res. 256 50-57.

 C.S. Atwood, S.V. Meethal, T. Liu, A.C. Wilson, M. Gallego, M.A. Smith, R.L. Bowen, Dysregulation of the hypothalamic-pituitary-gonadal axis with menopause and andropause promotes neurodegenerative senescence, J. Neuropathol. Exp. Neurol. 64 (2005) 93-103.

 I. Azcoitia, A. Sierra, L.M. Garcia-Segura, Estradiol prevents kainic acid- induced neuronal loss in the rat dentate gyrus, Neuroreport 9 (1998) 30753079.

 I. Azcoitia, A. Sierra, L.M. Garcia_Segura, Neuroprotective effects of estradiol in the adult rat hippocampus: interaction with insulin-like growth factor-I signalling., J. Neurosci. Res. 58 (1999) 815-822.

 I. Azcoitia, A. Sierra, S. Veiga, S. Honda, N. Harada, L.M. Garcia-Segura, Brain aromatase is neuroprotective, J. Neurobiol. 47 (2001) 318-329.

 D.L. Bachman, P.A. Wolf, R. Linn, J.E. Knoefel, J. Cobb, A. Belanger, R.B. D'Agostino, L.R. White, Prevalence of dementia and probable senile dementia of the Alzheimer type in the Framingham study, Neurology 42 (1992) 115119.

 S. Bake, F. Sohrabji, 17Beta-estradiol differentially regulates blood-brain barrier permeability in young and aging female rats, Endocrinology 145 5471-5475.

 S. Bake, L. Ma, F. Sohrabji, Estrogen receptor-alpha overexpression suppresses 17beta-estradiol-mediated vascular endothelial growth factor expression and activation of survival kinases, Endocrinology 149 (2008) 3881-3889.

 L.D. Baker, K. Sambamurti, S. Craft, M. Cherrier, M.A. Raskind, F.Z. Stanczyk, S.R. Plymate, S. Asthana, 17Beta-estradiol reduces plasma Abeta40 for HRT- naive postmenopausal women with Alzheimer disease: a preliminary study, Am J. Geriat. Psychiatry 11 (2003) 239-244.

 L.L. Barnes, R.S. Wilson, J.L. Bienias, J.A. Schneider, D.A. Evans, D.A. Bennett, Sex differences in the clinical manifestations of Alzheimer disease pathology, Arch. Gen. Psychiatry 62 (2005) 685-691.

 E. Barret-Connor, D. Kritz-Silverstein, Estrogen replacement therapy and cognitive function in older women, J. Am. Med. Assoc. 269 (1993) 2637-2641.

 E. Barrett-Connor, D. Goodman-Gruen, B. Patay, Endogenous sex hormones and cognitive function in older men, J. Clin. Endocrinol. Metab. 84 (1999) 3681-3685.

 R.N. Baumgartner, D.L. Waters, D. Gallagher, J.E. Morley, P.J. Garry, Predictors of skeletal muscle mass in elderly men and women, Mech. Ageing Dev. 107 123-136.

 C. Behl, J.B. Davis, R. Lesley, D. Schubert, Hydrogen peroxide mediates amyloid b protein toxicity, Cell 77 (1994) 817-827.

 C. Behl, M. Widmann, T. Trapp, F. Holsboer, 17-Beta estradiol protects neurons from oxidative stress-induced cell death in vitro, Biochem. Biophys. Res. Commun. 216 (1995) 473-482.

 C. Behl, T. Trapp, T. Skutella, F. Holsboer, Protection against oxidative stress- induced neuronal cell death - a novel role for RU486, Eur. J. Neurosci. 9 912-920.

 S. Benvenuti, P. Luciani, G.B. Vannelli, S. Gelmini, E. Franceschi, M. Serio, A. Peri, Estrogen and selective estrogen receptor modulators exert neuroprotective effects and stimulate the expression of selective Alzheimer's disease indicator-1, a recently discovered antiapoptotic gene, in human neuroblast long-term cell cultures, J. Clin. Endocrinol. Metab. 90 1775-1782.

 I. Bezprozvanny, M.P. Mattson, Neuronal calcium mishandling and the pathogenesis of Alzheimer's disease, Trends Neurosci. 31 (2008) 454-463.

 S. Bhasin, L. Woodhouse, R. Casaburi, A.B. Singh, D. Bhasin, N. Berman, X. Chen, K.E. Yarasheski, L. Magliano, C. Dzekov, J. Dzekov, R. Bross, J. Phillips, I. Sinha-Hikim, R. Shen, T.W. Storer, Testosterone dose-response relationships in healthy young men, Am. J. Physiol. Endocrinol. Metab. 281 (2001) E1172- 1181.

 S. Bhasin, G.R. Cunningham, F.J. Hayes, A.M. Matsumoto, P.J. Snyder, R.S. Swerdloff, V.M. Montori, Testosterone therapy in adult men with androgen deficiency syndromes: an endocrine society clinical practice guideline, J. Clin. Endocrinol. Metab. 91 (2006) 1995-2010.

 H.A. Bimonte-Nelson, M.E. Nelson, A.C. Granholm, Progesterone counteracts estrogen-induced increases in neurotrophins in the aged female rat brain, Neuroreport 15 (2004) 2659-2663.

 H.A. Bimonte-Nelson, K.R. Francis, C.D. Umphlet, A.C. Granholm, Progesterone reverses the spatial memory enhancements initiated by tonic and cyclic oestrogen therapy in middle-aged ovariectomized female rats, Eur. J. Neurosci. 24 (2006) 229-242.

 E.F. Binder, K.B. Schechtman, S.J. Birge, D.B. Williams, W.M. Kohrt, Effects of hormone replacement therapy on cognitive performance in elderly women, Maturitas 38 (2001) 137-146.

 J. Bohacek, A.M. Bearl, J.M. Daniel, Long-term ovarian hormone deprivation alters the ability of subsequent oestradiol replacement to regulate choline acetyltransferase protein levels in the hippocampus and prefrontal cortex of middle-aged rats, J. Neuroendocrinol. 20 (2008) 1023-1027.

 C.E. Bohl, D.D. Miller, J. Chen, C.E. Bell, J.T. Dalton, Structural basis for accommodation of nonsteroidal ligands in the androgen receptor, J. Biol. Chem. 280 (2005) 37747-37754.

 H. Braak, E. Braak, Alzheimer's disease: striatal amyloid deposits and neurofibrillary changes, J. Neuropathol. Exp. Neurol. 49 (1990) 215224.

 D.W. Brann, K. Dhandapani, C. Wakade, V.B. Mahesh, M.M. Khan, Neurotrophic and neuroprotective actions of estrogen: basic mechanisms and clinical implications, Steroids 72 (2007) 381-405.

 C. Brayne, C. Gill, F.A. Huppert, C. Barkley, E. Gehlhaar, D.M. Girling, D.W. O'Connor, E.S. Paykel, Incidence of clinically diagnosed subtypes of dementia in an elderly population. Cambridge Project for Later Life, Br. J. Psychiatry 167 (1995) 255-262.

 D.E. Brenner, W.A. Kukull, A. Stergachis, G. van Belle, J.D. Bowen, W.C. McCormick, L. Teri, E.B. Larson, Postmenopausal estrogen replacement therapy and the risk of Alzheimer's disease: a population-based case- control study, Am. J. Epidemiol. 140 (1994) 262-267.

 B. Breuer, R. Anderson, The relationship of tamoxifen with dementia, depression, and dependence in activities of daily living in elderly nursing home residents, Women Health 31 (2000) 71-85.

 R.D. Brinton, Requirements of a brain selective estrogen: advances and remaining challenges for developing a NeuroSERM, J. Alzheimers Dis. 6 (2004) S27-35.

 R.D. Brinton, S. Chen, M. Montoya, D. Hsieh, J. Minaya, The estrogen replacement therapy of the Women's Health Initiative promotes the cellular mechanisms of memory and neuronal survival in neurons vulnerable to Alzheimer's disease, Maturitas 34 (Suppl. 2) (2000) S35-52.

 R.D. Brinton, R.F. Thompson, M.R. Foy, M. Baudry, J. Wang, C.E. Finch, T.E. Morgan, C.J. Pike, W.J. Mack, F.Z. Stanczyk, J. Nilsen, Progesterone receptors: form and function in brain, Front. Neuroendocrinol. 29 (2008) 313-339.

 B.P. Brooks, D.E. Merry, H.L. Paulson, A.P. Lieberman, D.L. Kolson, K.H. Fischbeck, A cell culture model for androgen effects in motor neurons, J. Neurochem. 70 (1998) 1054-1060.

 T.R. Brown, Nonsteroidal selective androgen receptors modulators (SARMs): designer androgens with flexible structures provide clinical promise, Endocrinology 145 (2004) 5417-5419.

 A.J. Bruce-Keller, J.L. Keeling, J.N. Keller, F.F. Huang, S. Camondola, M.P. Mattson, Antiinflammatory effects of estrogen on microglial activation, Endocrinology 141 (2000) 3646-3656.

  H.U. Bryant, W.H. Dere, Selective estrogen receptor modulators: an alternative to hormone replacement therapy, Proc. Soc. Exp. Biol. Med. 217 45-52.

 J.G. Buckwalter, E. Sobel, M.E. Dunn, M.M. Diz, V.W. Henderson, Gender differences on a briefmeasure ofcognitive functioning in Alzheimer's disease, Arch. Neurol. 50 (1993) 757-760.

 H. Burger, C.E. de Laet, P.L. van Daele, A.E. Weel, J.C. Witteman, A. Hofman, H.A. Pols, Risk factors for increased bone loss in an elderly population: the Rotterdam study, Am. J. Epidemiol. 147 (1998) 871-879.

 M.J. Callahan, W.J. Lipinski, F. Bian, R.A. Durham, A. Pack, L.C. Walker, Augmented senile plaque load in aged female beta-amyloid precursor protein-transgenic mice, Am. J. Pathol. 158 (2001) 1173-1177.

 M.C. Carlson, P.P. Zandi, B.L. Plassman, J.T. Tschanz, K.A. Welsh-Bohmer, D.C. Steffens, L.A. Bastian, K.M. Mehta, J.C. Breitner, Hormone replacement therapy and reduced cognitive decline in older women: the Cache County Study, Neurology 57 (2001) 2210-2216.

 J.C. Carroll, C.J. Pike, Selective estrogen receptor modulators differentially regulate Alzheimer-like changes in female 3xTg-AD mice, Endocrinology 149 (2008)2607-2611.

 J.C. Carroll, E.R. Rosario, L. Chang, F.Z. Stanczyk, S. Oddo, F.M. LaFerla, C.J. Pike, Progesterone and estrogen regulate Alzheimer-like neuropathology in female 3xTg-AD mice, J. Neurosci. 27 (2007) 13357-13365.

 J.C. Carroll, E.R. Rosario, C.J. Pike, Progesterone blocks estrogen neuroprotection from kainate in middle-aged female rats, Neurosci. Lett. 445 (2008) 229-232.

 C. Castelo-Branco, F. Figueras, A. Sanjuan, F. Pons, J.J. Vicente, J.A. Vanrell, Long-term postmenopausal hormone replacement therapy effects on bone mass: differences between surgical and spontaneous patients, Eur. J. Obstet. Gynecol. Reprod. Biol. 83 (1999) 207-211.

 H.S. Chae, J.H. Bach, M.W. Lee, H.S. Kim, Y.S. Kim, K.Y. Kim, K.Y. Choo, S.H. Choi, C.H. Park, S.H. Lee, Y.H. Suh, S.S. Kim, W.B. Lee, Estrogen attenuates cell death induced by carboxy-terminal fragment of amyloid precursor protein in PC12 through a receptor-dependent pathway, J. Neurosci. Res. 65 (2001) 403-407.

 T.R. Chakraborty, A.C. Gore, Aging-related changes in ovarian hormones, their receptors, and neuroendocrine function, Exp. Biol. Med. (Maywood) 229 (2004)977-987.

 D. Chang, J. Kwan, P.S. Timiras, Estrogens influence growth, maturation, and amyloid beta-peptide production in neuroblastoma cells and in a beta-APP transfected kidney 293 cell line, Adv. Exp. Med. Biol. 429 (1997) 261-271.

 P. Chaovipoch, K.A. Jelks, L.M. Gerhold, E.J. West, S. Chongthammakun, C.L. Floyd, 17Beta-estradiol is protective in spinal cord injury in post- and premenopausal rats, J. Neurotrauma. 23 (2006) 830-852.

 I.H. Cheng, K. Scearce-Levie, J. Legleiter, J.J. Palop, H. Gerstein, N. Bien-Ly, J. Puolivali, S. Lesne, K.H. Ashe, P.J. Muchowski, L. Mucke, Accelerating amyloidbeta fibrillization reduces oligomer levels and functional deficits in Alzheimer disease mouse models, J. Biol. Chem. 282 (2007) 23818-23828.

 M.M. Cherrier, S. Asthana, S. Plymate, L. Baker, A.M. Matsumoto, E. Peskind, M.A. Raskind, K. Brodkin, W. Bremner, A. Petrova, S. LaTendresse, S. Craft, Testosterone supplementation improves spatial and verbal memory in healthy older men, Neurology 57 (2001) 80-88.

 M.M. Cherrier, A.M. Matsumoto, J.K. Amory, S. Asthana, W. Bremner, E.R. Peskind, M.A. Raskind, S. Craft, Testosterone improves spatial memory in men with Alzheimer disease and mild cognitive impairment, Neurology 64 (2005) 2063-2068.

 D.W. Choi, Ionic dependence of glutamate neurotoxicity, J. Neurosci. 7 (1987) 369-379.

 P. Ciana, G. Di Luccio, S. Belcredito, G. Pollio, E. Vegeto, L. Tatangelo, C. Tiveron, A. Maggi, Engineering of a mouse for the in vivo profiling of estrogen receptor activity, Mol. Endocrinol. 15 (2001) 1104-1113.

 E.H. Corder, E. Ghebremedhin, M.G. Taylor, D.R. Thal, T.G. Ohm, H. Braak, The biphasic relationship between regional brain senile plaque and neurofibrillary tangle distributions: modification by age, sex, and APOE polymorphism, Ann. NY Acad. Sci. 1019 (2004) 24-28.

 M. Cordey, C.J. Pike, Neuroprotective properties of selective estrogen receptor agonists in cultured neurons, Brain Res. 1045 (2005) 217-223.

 M. Cordey, C.J. Pike, Conventional protein kinase C isoforms mediate neuroprotection induced by phorbol ester and estrogen, J. Neurochem. 96 204-217.

 M. Cordey, U. Gundimeda, R. Gopalakrishna, C.J. Pike, Estrogen activates protein kinase C in neurons: role in neuroprotection, J. Neurochem. 84 (2003) 1340-1348.

 S. Cory, J.M. Adams, The Bcl2 family: regulators of the cellular life-or-death switch, Nat. Rev. Cancer 2 (2002) 647-656.

 C.W. Cotman, A.J. Anderson, A potential role for apoptosis in neurodegeneration and Alzheimer's disease, Mol. Neurobiol. 10 (1995) 1945.

 C.W. Cotman, C.J. Pike, A. Copani, Beta-Amyloid neurotoxicity: a discussion of in vitro findings, Neurobiol. Aging 13 (1992) 587-590.

 C.W. Cotman, D.H. Cribbs, C.J. Pike, K.J. Ivins, Cell death in Alzheimer's death. When Cells Die: A Comprehensive Evaluation of Apoptosis and Programmed Cell Death, 1998, pp. 385-409.

 C.M. Coughlan, K.C. Breen, Factors influencing the processing and function of the amyloid beta precursor protein — a potential therapeutic target in Alzheimer's disease?, Pharmacol Ther. 86 (2000) 111-144.

 M.C. Craig, P.M. Maki, D.G. Murphy, The Women's Health Initiative memory study: findings and implications for treatment, Lancet Neurol. 4 (2005) 190194.

 D.E. Cummings, N. Kumar, C.W. Bardin, K. Sundaram, W.J. Bremner, Prostatesparing effects in primates of the potent androgen 7alpha-methyl-19- nortestosterone: a potential alternative to testosterone for androgen replacement and male contraception, J. Clin. Endocrinol. Metab. 83 (1998) 4212-4219.

 D. Dai, D.M. Wolf, E.S. Litman, M.J. White, K.K. Leslie, Progesterone inhibits human endometrial cancer cell growth and invasiveness: down-regulation of cellular adhesion molecules through progesterone B receptors, Cancer Res. 62 881-886.

 J.M. Daniel, J.L. Hulst, J.L. Berbling, Estradiol replacement enhances working memory in middle-aged rats when initiated immediately after ovariectomy but not after a long-term period of ovarian hormone deprivation, Endocrinology 147 (2006) 607-614.

 S. Davies, D. Dai, D.M. Wolf, K.K. Leslie, Immunomodulatory and transcriptional effects of progesterone through progesterone A and B receptors in Hec50co poorly differentiated endometrial cancer cells, J. Soc. Gynecol. Investig. 11 (2004) 494-499.

 S. Del Cerro, J. Garcia-Estrada, L.M. Garcia-Segura, Neuroactive steroids regulate astroglia morphology in hippocampal cultures from adult rats, Glia 14 (1995) 65-71.

 P.D. Delmas, N.H. Bjarnason, B.H. Mitlak, A.C. Ravoux, A.S. Shah, W.J. Huster, M. Draper, C. Christiansen, Effects of raloxifene on bone mineral density, serum cholesterol concentrations, and uterine endometrium in postmenopausal women, New Engl. J. Med. 337 (1997) 1641-1647.

 D.E. Dluzen, Effects of testosterone upon MPTP-induced neurotoxicity of the nigrostriatal dopaminergic system of C57/B1 mice, Brain Res. 715 (1996) 113-118.

 L. Dong, W. Wang, F. Wang, M. Stoner, J.C. Reed, M. Harigai, I. Samudio, M.P. Kladde, C. Vyhlidal, S. Safe, Mechanisms of transcriptional activation of Bcl-2

 gene expression by 17beta-estradiol in breast cancer cells, J. Biol. Chem. 274 32099-32107.

 R. Duara, W.W. Barker, R. Lopez-Alberola, D.A. Loewenstein, L.B. Grau, D. Gilchrist, S. Sevush, S. St George-Hyslop, Alzheimer's disease: interaction of apolipoprotein E genotype, family history of dementia, gender, education, ethnicity, and age of onset, Neurology 46 (1996) 1575-1579.

 D.B. Dubal, P.M. Wise, Neuroprotective effects of estradiol in middle-aged female rats, Endocrinology 142 (2001) 43-48.

 D.B. Dubal, P.J. Shughrue, M.E. Wilson, I. Merchenthaler, P.M. Wise, Estradiol modulates Bcl-2 in cerebral ischemia: a potential role for estrogen receptors, J. Neurosci. 19 (1999) 6385-6393.

  K.L. Edinger, C.A. Frye, Testosterone's analgesic, anxiolytic, and cognitive-enhancing effects may be due in part to actions of its 5alpha-reduced metabolites in the hippocampus, Behav. Neurosci. 118 1352-1364.

 K.L. Edinger, C.A. Frye, Testosterone's anti-anxiety and analgesic effects may be due in part to actions of its 5alpha-reduced metabolites in the hippocampus, Psychoneuroendocrinology 30 (2005) 418-430.

 J. El Khoury, S.E. Hickman, C.A. Thomas, L. Cao, S.C. Silverstein, J.D. Loike, Scavenger receptor-mediated adhesion of microglia to beta-amyloid fibrils, Nature 382 (1996) 716-719.

 M.H. Emmelot-Vonk, H.J. Verhaar, H.R. Nakhai Pour, A. Aleman, T.M. Lock, J.L. Bosch, D.E. Grobbee, Y.T. van der Schouw, Effect of testosterone supplementation on functional mobility, cognition, and other parameters in older men: a randomized controlled trial, Jama 299 (2008) 39-52.

 M.A. Espeland, S.R. Rapp, S.A. Shumaker, R. Brunner, J.E. Manson, B.B. Sherwin, J. Hsia, K.L. Margolis, P.E. Hogan, R. Wallace, M. Dailey, R. Freeman, J. Hays, Conjugated equine estrogens and global cognitive function in postmenopausal women: Women's Health Initiative Memory Study, Jama 291 (2004) 2959-2968.

 M. Estrada, P. Uhlen, B.E. Ehrlich, Ca²⁺ oscillations induced by testosterone enhance neurite outgrowth, J. Cell Sci. 119 (2006) 733-743.

 L.A. Farrer, L.A. Cupples, J.L. Haines, B. Hyman, W.A. Kukull, R. Mayeux, R.H. Myers, M.A. Pericak-Vance, N. Risch, C.M. van Duijn, Effects of age, sex, and ethnicity on the association between apolipoprotein E genotype and Alzheimer disease. A meta-analysis. APOE and Alzheimer Disease Meta Analysis Consortium, Jama 278 (1997) 1349-1356.

 H.A. Feldman, C. Longcope, C.A. Derby, C.B. Johannes, A.B. Araujo, A.D. Coviello, W.J. Bremner, J.B. McKinlay, Age trends in the level of serum testosterone and other hormones in middle-aged men: longitudinal results from the Massachusetts male aging study, J. Clin. Endocrinol. Metab. 87 589-598.

 L.S. Felicio, J.F. Nelson, C.E. Finch, Longitudinal studies of estrous cyclicity in aging C57BL/6J mice: II. Cessation of cyclicity and the duration of persistent vaginal cornification, Biol. Reprod. 31 (1984) 446-453.

 A.A. Ferrando, M. Sheffield-Moore, C.W. Yeckel, C. Gilkison, J. Jiang, A. Achacosa, S.A. Lieberman, K. Tipton, R.R. Wolfe, R.J. Urban, Testosterone administration to older men improves muscle function: molecular and physiological mechanisms, Am. J. Physiol. Endocrinol. Metab. 282 (2002) E601-607.

 J.L. Fitzpatrick, A.L. Mize, C.B. Wade, J.A. Harris, R.A. Shapiro, D.M. Dorsa, Estrogen-mediated neuroprotection against beta-amyloid toxicity requires expression of estrogen receptor alpha or beta and activation of the MAPK pathway, J. Neurochem. 82 (2002) 674-682.

 A. Fleisher, M. Grundman, C.R. Jack Jr., R.C. Petersen, C. Taylor, H.T. Kim, D.H. Schiller, V. Bagwell, D. Sencakova, M.F. Weiner, C. DeCarli, S.T. DeKosky, C.H. van Dyck, L.J. Thal, Sex, apolipoprotein E epsilon 4 status, and hippocampal volume in mild cognitive impairment, Arch. Neurol. 62 953-957.

 N. Fleshner, A.R. Zlotta, Prostate cancer prevention: past, present, and future, Cancer 110 (2007) 1889-1899.

 M. Frankfurt, E. Gould, C.S. Woolley, B.S. McEwen, Gonadal steroids modify dendritic spine density in ventromedial hypothalamic neurons: a Golgi study in the adult rat, Neuroendocrinology 51 (1990) 530-535.

 L. Fratiglioni, M. Viitanen, E. von Strauss, V. Tontodonati, A. Herlitz, B. Winblad, Very old women at highest risk of dementia and Alzheimer's disease: incidence data from the Kungsholmen Project, Stockholm, Neurology 48 (1997) 132-138.

 C.A. Frye, T.A. Reed, Androgenic neurosteroids: anti-seizure effects in an animal model of epilepsy, Psychoneuroendocrinology 23 (1998) 385-399.

 C.A. Frye, A.M. Seliga, Testosterone increases analgesia, anxiolysis, and cognitive performance of male rats, Cogn. Affect. Behav. Neurosci. 1 (2001) 371-381.

 C.A. Frye, A.A. Walf, Effects of progesterone administration and APP_swe+PSEN1Deltae9 mutation for cognitive performance of mid-aged mice, Neurobiol. Learn. Mem. 89 (2008) 17-26.

 E. Fukuzaki, K. Takuma, Y. Funatsu, Y. Himeno, Y. Kitahara, B. Gu, H. Mizoguchi, D. Ibi, K. Koike, M. Inoue, S.D. Yan, K. Yamada, Ovariectomy increases neuronal amyloid-beta binding alcohol dehydrogenase level in the mouse hippocampus, Neurochem. Int. 52 (2008) 1358-1364.

 S. Gandy, S. Petanceska, Regulation of alzheimer beta-amyloid precursor trafficking and metabolism, Adv. Exp. Med. Biol. 487 (2001) 85-100.

 S. Gandy, O.P. Almeida, J. Fonte, D. Lim, A. Waterrus, N. Spry, L. Flicker, R.N. Martins, Chemical andropause and amyloid-beta peptide, J. Am. Med. Assoc. 285 (2001) 2195-2196.

 W. Gao, P.J. Reiser, C.C. Coss, M.A. Phelps, J.D. Kearbey, D.D. Miller, J.T. Dalton, Selective androgen receptor modulator treatment improves muscle strength and body composition and prevents bone loss in orchidectomized rats, Endocrinology 146 (2005) 4887-4897.

 J. Garcia-Estrada, J.A. Del Rio, S. Luquin, E. Soriano, L.M. Garcia-Segura, Gonadal hormones down-regulate reactive gliosis and astrocyte proliferation after a penetrating brain injury, Brain Res. 628 (1993) 271-278.

 L.M. Garcia-Segura, P. Cardona-Gomez, F. Naftolin, J.A. Chowen, Estradiol upregulates Bcl-2 expression in adult brain neurons, Neuroreport 9 (1998) 593-597.

 L.M. Garcia-Segura, A. Wozniak, I. Azcoitia, J.R. Rodriguez, R.E. Hutchison, J.B. Hutchison, Aromatase expression by astrocytes after brain injury: implications for local estrogen formation in brain repair, Neuroscience 89 (1999) 567-578.

 J.W. Gatson, M. Singh, Activation of a membrane-associated androgen receptor promotes cell death in primary cortical astrocytes, Endocrinology 148 (2007) 2458-2464.

 J.W. Gatson, P. Kaur, M. Singh, Dihydrotestosterone differentially modulates the mitogen-activated protein kinase and the phosphoinositide 3-kinase/Akt pathways through the nuclear and novel membrane androgen receptor in C6 cells, Endocrinology 147 (2006) 2028-2034.

 D.M. Gee, K. Flurkey, C.E. Finch, Aging and the regulation of luteinizing hormone in C57BL/6J mice: impaired elevations after ovariectomy and spontaneous elevations at advanced ages, Biol. Reprod. 28 (1983) 598-607.

 R.B. Gibbs, Effects of gonadal hormone replacement on measures of basal forebrain cholinergic function, Neuroscience 101 (2000) 931-938.

 R.B. Gibbs, Oestrogen and the cholinergic hypothesis: implications for oestrogen replacement therapy in postmenopausal women, Novartis Found Symp 230 (2000) 94-107; discussion 107-111.

 M.J. Gillett, R.N. Martins, R.M. Clarnette, S.A. Chubb, D.G. Bruce, B.B. Yeap, Relationship between testosterone, sex hormone binding globulin and plasma amyloid beta peptide 40 in older men with subjective memory loss or dementia, J. Alzheimers Dis. 5 (2003) 267-269.

 C.E. Gleason, C.M. Carlsson, S. Johnson, C. Atwood, S. Asthana, Clinical pharmacology and differential cognitive efficacy of estrogen preparations, Ann. NY Acad. Sci. 1052 (2005) 93-115.

 J.A. Goebel, S.J. Birge, S.C. Price, J.M. Hanson, D.G. Fishel, Estrogen replacement therapy and postural stability in the elderly, Am. J. Otolaryng. 16(1995)470-474.

 M.S. Golub, S.L. Germann, M. Mercer, M.N. Gordon, D.G. Morgan, L.P. Mayer, P.B. Hoyer, Behavioral consequences of ovarian atrophy and estrogen replacement in the APP_swe mouse, Neurobiol. Aging 29 (2007) 1512-1523.

 S. Goodenough, S. Engert, C. Behl, Testosterone stimulates rapid secretory amyloid precursor protein release from rat hypothalamic cells via the activation of the mitogen-activated protein kinase pathway, Neurosci. Lett. 296 (2000) 49-52.

 S. Goodenough, D. Schleusner, C. Pietrzik, T. Skutella, C. Behl, Glycogen synthase kinase 3beta links neuroprotection by 17beta-estradiol to key Alzheimer processes, Neuroscience 132 (2005) 581-589.

 Y. Goodman, M.P. Mattson, Secreted forms of b-amyloid precursor protein protect hippocampal neurons against amyloid b-peptide-induced oxidative injury, Exp. Neurol. 128 (1994) 1-12.

 Y. Goodman, A.J. Bruce, B. Cheng, M.P. Mattson, Estrogens attenuate and corticosterone exacerbates excitotoxicity, oxidative injury, and amyloid betapeptide toxicity in hippocampal neurons, J. Neurochem. 66 (1996) 18361844.

 L. Gooren, Androgen deficiency in the aging male: benefits and risks of androgen supplementation, J. Steroid. Biochem. Mol. Biol. 85 (2003) 349-355.

 R.G. Gosden, S.C. Laing, L.S. Felicio, J.F. Nelson, C.E. Finch, Imminent oocyte exhaustion and reduced follicular recruitment mark the transition to acyclicity in aging C57BL/6J mice, Biol. Reprod. 28 (1983) 255-260.

 C. Gouchie, D. Kimura, The relationship between testosterone levels and cognitive ability patterns, Psychoneuroendocrinology 16 (1991) 323-334.

 G.K. Gouras, H. Xu, R.S. Gross, J.P. Greenfield, B. Hai, R. Wang, P. Greengard, Testosterone reduces neuronal secretion of Alzheimer's beta-amyloid peptides, PNAS 97 (2000) 1202-1205.

 D. Grady, T. Gebretsadik, K. Kerlikowske, V. Ernster, D. Petitti, Hormone replacement therapy and endometrial cancer risk: a meta-analysis, Obstet. Gynecol. 85 (1995) 304-313.

 J.D. Graham, C.L. Clarke, Physiological action of progesterone in target tissues, Endocr. Rev. 18 (1997) 502-519.

 A. Gray, H. Feldman, J. McKinlay, C. Longcope, Age, disease, and changing sex hormone levels in middle-aged men: results of the Massachusetts male aging study, J. Clin. Endocrinol. Metab. 73 (1991) 1016-1025.

 P.S. Green, K.E. Gridley, J.W. Simpkins, Estradiol protects against betaamyloid (25-35)-induced toxicity in SK-N-SH human neuroblastoma cells, Neurosci. Lett. 218 (1996) 165-168.

 P.S. Green, K. Bales, S. Paul, G.J. Bu, Estrogen therapy fails to alter amyloid deposition in the PDAPP model of Alzheimer's disease, Endocrinology 146 (2005) 2774-2781.

 J.P. Greenfield, L.W. Leung, D. Cai, K. Kaasik, R.S. Gross, E. Rodriguez_Boulan, P. Greengard, H. Xu, Estrogen lowers Alzheimer beta-amyloid generation by stimulating trans-Golgi network vesicle biogenesis, J. Biol. Chem. 277 (2002) 12128-12136.

 K.E. Gridley, P.S. Green, J.W. Simpkins, Low concentrations ofestradiol reduce b-amyloid (25-35)-induced toxicity, lipid peroxidation and glucose utilization in human SK-N-SH neuroblastoma cells, Brain Res. 778 (1997) 158-165.

 C. Haass, D.J. Selkoe, Soluble protein oligomers in neurodegeneration: lessons from the Alzheimer's amyloid beta-peptide, Nat. Rev. Mol. Cell Biol. 8 (2007) 101-112.

 O. Hagnell, L. Ojesjo, B. Rorsman, Incidence of dementia in the Lundby study, Neuroepidemiology 11 (Suppl. 1) (1992) 61-66.

 J. Hammond, Q. Le, C. Goodyer, M. Gelfand, M. Trifiro, A. LeBlanc, Testosterone-mediated neuroprotection through the androgen receptor in human primary neurons, J. Neurochem. 77 (2001) 1319-1326.

 J. Hardy, The Alzheimer family of diseases: many etiologies, one pathogenesis?, Proc Natl. Acad. Sci. USA 94 (1997) 2095-2097.

 J.A. Hardy, G.A. Higgins, Alzheimer's disease: the amyloid cascade hypothesis, Science 256 (1992) 184-185.

 M.T. Haren, G.A. Wittert, I.M. Chapman, P. Coates, J.E. Morley, Effect of oral testosterone undecanoate on visuospatial cognition, mood and quality of life in elderly men with low-normal gonadal status, Maturitas 50 (2005) 124133.

 M.T. Haren, M.J. Kim, S.H. Tariq, G.A. Wittert, J.E. Morley, Andropause: a quality-of-life issue in older males, Med. Clin. North Am. 90 (2006) 10051023.

 M.E. Harris-White, T. Chu, S.A. Miller, M. Simmons, B. Teter, D. Nash, G.M. Cole, S.A. Frautschy, Estrogen (E2) and glucocorticoid (Gc) effects on microglia and Ab clearance in vitro and in vivo, Neurochem. Int. 39 (2001) 435-448.

 S.A. Hart, J.D. Patton, C.S. Woolley, Quantitative analysis of ERalpha and GAD colocalization in the hippocampus of the adult female rat, J. Comp. Neurol. 440 (2001) 144-155.

 D.M. Hartley, D.M. Walsh, C.P. Ye, T. Diehl, S. Vasquez, P.M. Vassilev, D.B. Teplow, D.J. Selkoe, Protofibrillar intermediates of amyloid beta-protein induce acute electrophysiological changes and progressive neurotoxicity in cortical neurons, J. Neurosci. 19 (1999) 8876-8884.

 S.G. Haskell, E.D. Richardson, R.I. Horwitz, The effect of estrogen replacement therapy on cognitive function in women: a critical review of the literature, J. Clin. Epidemiol. 50 (1997) 1249-1264.

 B. He, J.T. Minges, L.W. Lee, E.M. Wilson, The FXXLF motif mediates androgen receptor-specific interactions with coregulators, J. Biol. Chem. 277 (2002) 10226-10235.

 T. Heikkinen, G. Kalesnykas, A. Rissanen, T. Tapiola, S. Iivonen, J. Wang, J. Chaudhuri, H. Tanila, R. Miettinen, J. Puolivali, Estrogen treatment improves spatial learning in APP + PS1 mice but does not affect beta amyloid accumulation and plaque formation, Exp. Neurol. 187 (2004) 105-117.

 V.W. Henderson, Estrogen-containing hormone therapy and Alzheimer's disease risk: understanding discrepant inferences from observational and experimental research, Neuroscience 138 (2006) 1031-1039.

 V. Henderson, J. Buckwalter, Cognitive deficits of men and women with Alzheimer's disease, Neurology 44 (1994) 90-96.

 V.W. Henderson, A. Paganini-Hill, C.K. Emanuel, M.E. Dunn, J.G. Buckwalter, Estrogen replacement therapy in older women. Comparisons between Alzheimer's disease cases and nondemented control subjects, Arch. Neurol. 51 (1994) 896-900.

 V.W. Henderson, A. Paganini_Hill, B.L. Miller, R.J. Elble, P.F. Reyes, D. Shoupe, C.A. McCleary, R.A. Klein, A.M. Hake, M.R. Farlow, Estrogen for Alzheimer's disease in women: randomized, double-blind, placebo-controlled trial, Neurology 54 (2000) 295-301.

 V.W. Henderson, J.R. Guthrie, E.C. Dudley, H.G. Burger, L. Dennerstein, Estrogen exposures and memory at midlife: a population-based study of women, Neurology 60 (2003) 1369-1371.

 V.W. Henderson, K.S. Benke, R.C. Green, L.A. Cupples, L.A. Farrer, Postmenopausal hormone therapy and Alzheimer's disease risk: interaction with age, J. Neurol. Neurosurg. Psychiatry 76 (2005) 103-105.

 A. Hestiantoro, D.F. Swaab, Changes in estrogen receptor-alpha and -beta in the infundibular nucleus of the human hypothalamus are related to the occurrence of Alzheimer's disease neuropathology, J. Clin. Endocrinol. Metab. 89 (2004) 1912-1925.

 C. Hirata-Fukae, H.F. Li, H.S. Hoe, A.J. Gray, S.S. Minami, K. Hamada, T. Niikura, F. Hua, H. Tsukagoshi-Nagai, Y. Horikoshi-Sakuraba, M. Mughal, G.W. Rebeck, F.M. LaFerla, M.P. Mattson, N. Iwata, T.C. Saido, W.L. Klein, K.E. Duff, P.S. Aisen, Y. Matsuoka, Females exhibit more extensive amyloid, but not tau, pathology in an Alzheimer transgenic model, Brain Res. 1216 (2008) 92103.

 M.A. Hlatky, D. Boothroyd, E. Vittinghoff, P. Sharp, M.A. Whooley, Quality-of- life and depressive symptoms in postmenopausal women after receiving hormone therapy: results from the Heart and Estrogen/Progestin Replacement Study (HERS) trial, Jama 287 (2002) 591-597.

 E. Hogervorst, M. Boshuisen, W. Riedel, C. Willeken, J. Jolles, 1998 Curt P. Richter Award. The effect of hormone replacement therapy on cognitive function in elderly women, Psychoneuroendocrinology 24 (1999) 43-68.

 E. Hogervorst, J. Williams, M. Biudge, L. Barnetson, M. Combrinck, A.D. Smith, Serum total testosterone is lower in men with Alzheimer's disease, Neuroendocrinol. Lett. 22 (2001) 163-168.

 E. Hogervorst, D.J. Lehmann, D.R. Warden, J. McBroom, A.D. Smith, Apolipoprotein E epsilon4 and testosterone interact in the risk of Alzheimer's disease in men, Int. J. Geriatr. Psych. 17 (2002) 938-940.

 E. Hogervorst, M. Combrinck, A.D. Smith, Testosterone and gonadotropin levels in men with dementia, Neuroendocrinol. Lett. 24 (2003) 203-208.

 H. Honjo, K. Iwasa, M. Kawata, S. Fushiki, T. Hosoda, H. Tatsumi, N. Oida, M. Mihara, Y. Hirasugi, H. Yamamoto, N. Kikuchi, J. Kitawaki, Progestins and estrogens and Alzheimer's disease, J. Steroid. Biochem. Mol. Biol. 93 (2005) 305-308.

 J. Huang, H. Guan, R.M. Booze, C.B. Eckman, L.B. Hersh, Estrogen regulates neprilysin activity in rat brain, Neurosci. Lett. 367 (2004) 85-87.

 C.B. Huppenbauer, L. Tanzer, L.L DonCarlos, K.J. Jones, Gonadal steroid attenuation of developing hamster facial motoneuron loss by axotomy: equal efficacy of testosterone, dihydrotestosterone, and 17-beta estradiol, J. Neurosci. 25 (2005) 4004-4013.

 B.T. Hyman, T. Gomez-Isla, M. Briggs, H. Chung, S. Nichols, F. Kohout, R. Wallace, Apolipoprotein E and cognitive change in an elderly population, Ann. Neurol. 40 (1996) 55-66.

 K. Iqbal, C. Alonso Adel, S. Chen, M.O. Chohan, E. El-Akkad, C.X. Gong, S. Khatoon, B. Li, F. Liu, A. Rahman, H. Tanimukai, I. Grundke-Iqbal, Tau pathology in Alzheimer disease and other tauopathies, Biochim. Biophys. Acta 1739 (2005) 198-210.

 R.W. Irwin, J. Yao, R.T. Hamilton, E. Cadenas, R.D. Brinton, J. Nilsen, Progesterone and estrogen regulate oxidative metabolism in brain mitochondria, Endocrinology 149 (2008) 3167-3175.

 U. Islander, M.C. Erlandsson, B. Hasseus, C.A. Jonsson, C. Ohlsson, J.A. Gustafsson, U. Dahlgren, H. Carlsten, Influence of oestrogen receptor alpha and beta on the immune system in aged female mice, Immunology 110 149-157.

 K.J. Ivins, E.T. Bui, C.W. Cotman, Beta-amyloid induces local neurite degeneration in cultured hippocampal neurons: evidence for neuritic apoptosis, Neurobiol. Dis. 5 (1998) 365-378.

 N. Iwata, S. Tsubuki, Y. Takaki, K. Watanabe, M. Sekiguchi, E. Hosoki, M. Kawashima-Morishima, H.J. Lee, E. Hama, Y. Sekine-Aizawa, T.C. Saido, Identification of the major Abeta1-42-degrading catabolic pathway in brain parenchyma: suppression leads to biochemical and pathological deposition, Nat. Med. 6 (2000) 143-150.

 A.B. Jaffe, C.D. Toran_Allerand, P. Greengard, S.E. Gandy, Estrogen regulates metabolism of Alzheimer amyloid beta precursor protein, J. Biol. Chem. 269 13065-13068.

 J.S. Janowsky, S.K. Oviatt, E.S. Orwoll, Testosterone influences spatial cognition in older men, Behav. Neurosci. 108 (1994) 325-332.

 J.S. Janowsky, B. Chavez, E. Orwoll, Sex steroids modify working memory, J. Cogn. Neurosci. 12 (2000) 407-414.

 A. Jayaraman, C.J. Pike, Progesterone attenuates oestrogen neuroprotection via downregulation of oestrogen receptor expression in cultured neurones, J. Neuroendocrinol. 21 (2009) 77-81.

 M.K. Jezierski, F. Sohrabji, Neurotrophin expression in the reproductively senescent forebrain is refractory to estrogen stimulation, Neurobiol. Aging 22 309-319.

 A.B. Johnson, S. Bake, D.K. Lewis, F. Sohrabji, Temporal expression ofIL-1beta protein and mRNA in the brain after systemic LPS injection is affected by age and estrogen, J. Neuroimmunol. 174 (2006) 82-91.

 K.J. Jones, S. Coers, P.D. Storer, L. Tanzer, N.B. Kinderman, Androgenic regulation of the central glia response following nerve damage, J. Neurobiol. 40 (1999) 560-573.

 K.J. Jones, T.J. Brown, M. Damaser, Neuroprotective effects of gonadal steroids on regenerating peripheral motoneurons, Brain Res. Brain Res. Rev. 37 (2001) 372-382.

 R.D. Jones, P.J. Pugh, J. Hall, K.S. Channer, T.H. Jones, Altered circulating hormone levels, endothelial function and vascular reactivity in the testicular feminised mouse, Eur. J. Endocrinol./Eur. Fed. Endocr. Soc. 148 (2003) 111120.

 A.F. Jorm, A.E. Korten, A.S. Henderson, The prevalence of dementia: a quantitative integration of the literature, Acta Psychiatr. Scand. 76 (1987) 465-479.

 G. Kalesnykas, U. Roschier, J. Puolivali, J. Wang, R. Miettinen, The effect of aging on the subcellular distribution of estrogen receptor-alpha in the cholinergic neurons of transgenic and wild-type mice, Eur. J. Neurosci. 21 (2005) 1437-1442.

 F. Kamenetz, T. Tomita, H. Hsieh, G. Seabrook, D. Borchelt, T. Iwatsubo, S. Sisodia, R. Malinow, APP processing and synaptic function, Neuron 37 (2003) 925-937.

 J.M. Kaufman, A. Vermeulen, The decline of androgen levels in elderly men and its clinical and therapeutic implications, Endocr. Rev. (2005).

 C. Kawas, S. Resnick, A. Morrison, R. Brookmeyer, M. Corrada, A. Zonderman, C. Bacal, D. Donnell Lingle, E. Metter, A prospective study of estrogen replacement therapy and the risk of developing Alzheimer's disease: the Baltimore longitudinal study of aging, Neurology 48 (1997) 1517-1521.

 J.F. Kelly, J.L. Bienias, A. Shah, K.A. Meeke, J.A. Schneider, E. Soriano, D.A. Bennett, Levels of estrogen receptors alpha and beta in frontal cortex of patients with Alzheimer's disease: relationship to Mini-Mental State Examination scores, Curr. Alzheimer Res. 5 (2008) 45-51.

 E.J. Kilbourne, W.J. Moore, L.P. Freedman, S. Nagpal, Selective androgen receptor modulators for frailty and osteoporosis, Curr. Opin. Investig. Drugs 8 821-829.

 H. Kim, O.Y. Bang, M.W. Jung, S.D. Ha, H.S. Hong, K. Huh, S.U. Kim, I. Mook- Jung, Neuroprotective effects of estrogen against beta-amyloid toxicity are mediated by estrogen receptors in cultured neuronal cells, Neurosci. Lett. 302 (2001) 58-62.

 J. Kim, D. Wu, D.J. Hwang, D.D. Miller,J.T. Dalton, The para substituent of S-3- (phenoxy)-2-hydroxy-2-methyl-N-(4-nitro-3-trifluoromethyl-phenyl)-prop ionamides is a major structural determinant of in vivo disposition and activity of selective androgen receptor modulators, J. Pharmacol. Exp. Ther. 315(2005)230-239.

 J.Y. Koh, L.L. Yang, C.W. Cotman, Beta-amyloid protein increases the vulnerability of cultured cortical neurons to excitotoxic damage, Brain Res. 533 (1990) 315-320.

 E. Koo, Phorbol esters affect multiple steps in beta-amyloid precursor protein trafficking and amyloid beta-protein production, Mol. Med. 3 (1997) 204211.

 C.L Koski, S. Hila, G.E. Hoffman, Regulation of cytokine-induced neuron death by ovarian hormones: involvement of antiapoptotic protein expression and c-JUN N-terminal kinase-mediated proapoptotic signaling, Endocrinology 145 (2004) 95-103.

 K.A. Kujawa, N.B. Kinderman, K.J.Jones, Testosterone-induced acceleration of recovery from facial paralysis following crush axotomy of the facial nerve in male hamsters, Exp. Neurol. 105 (1989) 80-85.

 K.A. Kujawa, E. Emeric, K.J. Jones, Testosterone differentially regulates the regenerative properties of injured hamster facial motoneurons, J. Neurosci. 11 (1991) 3898-3906.

 K.A. Kujawa, J.M.Jacob, K.J.Jones, Testosterone regulation of the regenerative properties of injured rat sciatic motor neurons, J. Neurosci. Res. 35 (1993) 268-273.

 K.A. Kujawa, L. Tanzer, K.J. Jones, Inhibition of the accelerative effects of testosterone on hamster facial nerve regeneration by the antiandrogen flutamide, Exp. Neurol. 133 (1995) 138-143.

 F.M. LaFerla, S. Oddo, Alzheimer's disease: Abeta, tau and synaptic dysfunction, Trends Mol. Med. 11 (2005) 170-176.

 E.S. LeBlanc, J. Janowsky, B.K.S. Chan, H.D. Nelson, Hormone replacement therapy and cognition: systematic review and meta-analysis, J. Am. Med. Assoc. 285 (2001) 1489.

 S. Lesne, M.T. Koh, L. Kotilinek, R. Kayed, C.G. Glabe, A. Yang, M. Gallagher, K.H. Ashe, A specific amyloid-beta protein assembly in the brain impairs memory, Nature 440 (2006) 352-357.

 J.A. Levin-Allerhand, C.E. Lominska,J. Wang,J.D. Smith, 17Alpha-estradiol and 17beta-estradiol treatments are effective in lowering cerebral amyloid-beta levels in AbetaPPSWE transgenic mice, J. Alzheimers Dis. 4 (2002) 449457.

 R. Li, Y. Shen, L.B. Yang, L.F. Lue, C. Finch, J. Rogers, Estrogen enhances uptake of amyloid beta-protein by microglia derived from the human cortex, J. Neurochem. 75 (2000) 1447-1454.

 J.J. Li, J.C. Sutton, A. Nirschl, Y. Zou, H. Wang, C. Sun, Z. Pi, R. Johnson, S.R. Krystek Jr., R. Seethala, R. Golla, P.G. Sleph, B.C. Beehler, G.J. Grover, A. Fura, V.P. Vyas, C.Y. Li, J.Z. Gougoutas, M.A. Galella, R. Zahler, J. Ostrowski, L.G. Hamann, Discovery of potent and muscle selective androgen receptor modulators through Scaffold modifications, J. Med. Chem. 50 (2007) 30153025.

 J.G. Liehr, D. Roy, Free radical generation by redox cycling of estrogens, Free Rad. Biol. Med. 8 (1990) 415-423.

 N. Linford, C. Wade, D. Dorsa, The rapid effects of estrogen are implicated in estrogen-mediated neuroprotection, J. Neurocytol. 29 (2000) 367374.

 X.A. Liu, L.Q. Zhu, Q. Zhang, H.R. Shi, S.H. Wang, Q. Wang, J.Z. Wang, Estradiol attenuates tau hyperphosphorylation induced by upregulation of protein kinase-A, Neurochem. Res. 33 (2008) 1811-1820.

 Y.P. Lu, M. Zeng, D.F. Swaab, R. Ravid, J.N. Zhou, Colocalization and alteration of estrogen receptor-alpha and -beta in the hippocampus in Alzheimer's disease, Hum. Pathol. 35 (2004) 275-280.

 P.H. Lu, D.A. Masterman, R. Mulnard, C. Cotman, B. Miller, K. Yaffe, E. Reback, V. Porter, R. Swerdloff, J.L. Cummings, Effects oftestosterone on cognition and mood in male patients with mild Alzheimer disease and healthy elderly men, Arch. Neurol. 63 (2006) 177-185.

 N.J. MacLusky, T. Hajszan, C. Leranth, Effects of dehydroepiandrosterone and flutamide on hippocampal CA1 spine synapse density in male and female rats: implications for the role of androgens in maintenance of hippocampal structure, Endocrinology 145 (2004) 4154-4161.

 N.J. MacLusky, T. Hajszan, J. Prange-Kiel, C. Leranth, Androgen modulation of hippocampal synaptic plasticity, Neuroscience 138 (2006) 957-965.

 P. Maki, Hormone therapy and risk for dementia: where do we go from here?, Gynecol Endocrinol. 19 (2003) 354.

 P.M. Maki, M. Ernst, E.D. London, K.L. Mordecai, P. Perschler, S.C. Durso, J. Brandt, A. Dobs, S.M. Resnick, Intramuscular testosterone treatment in elderly men: evidence of memory decline and altered brain function, J. Clin. Endocrinol. Metab. 92 (2007) 4107-4114.

 M.C. Manfredi, Y. Bi, A.A. Nirschl, J.C. Sutton, R. Seethala, R. Golla, B.C. Beehler, P.G. Sleph, G.J. Grover, J. Ostrowski, L.G. Hamann, Synthesis and SAR of tetrahydropyrrolo[1,2-b][1,2,5]thiadiazol-2(3H)-one 1,1-dioxide analogues as highly potent selective androgen receptor modulators, Bioorg. Med. Chem. Lett. 17 (2007) 4487-4490.

 J.J. Manly, C.A. Merchant, D.M. Jacobs, S. Small, K. Bell, M. Ferin, R. Mayeux, Endogenous estrogen levels and Alzheimer's disease among postmenopausal women, Neurology 54 (2000) 833-837.

 D. Manthey, S. Heck, S. Engert, C. Behl, Estrogen induces a rapid secretion of amyloid b precursor protein via the mitogen-activated protein kinase pathway, Eur. J. Biochem. 268 (2001) 4285-4291.

 R. Mark, K. Hensley, D. Butterfield, M. Mattson, Amyloid beta-peptide impairs ion-motive ATPase activities: evidence for a role in loss of neuronal Ca²⁺ homeostasis and cell death, J. Neurosci. 15 (1995) 6239-6249.

 M.P. Mattson, M.A. Lovell, K. Furukawa, W.R. Markesbery, Neurotrophic factors attenuate glutamate-induced accumulation of peroxides, elevation of intracellular Ca²⁺ concentration, and neurotoxicity and increase antioxidant enzyme activities in hippocampal neurons, J. Neurochem. 65 (1995) 17401751.

 M.P. Mattson, J. Partin, J.G. Begley, Amyloid beta-peptide induces apoptosis- related events in synapses and dendrites, Brain Res. 807 (1998) 167176.

 D.E. Meier, E.S. Orwoll, E.J. Keenan, R.M. Fagerstrom, Marked decline in trabecular bone mineral content in healthy men with age: lack of association with sex steroid levels, J. Am. Geriatr. Soc. 35 (1987) 189-197.

 A.L. Mize, L.J. Young, R.H. Alper, Uncoupling of 5-HT1A receptors in the brain by estrogens: regional variations in antagonism by ICI 182,780, Neuropharmacology 44 (2003) 584-591.

 S.D. Moffat, A.B. Zonderman, E.J. Metter, M.R. Blackman, S.M. Harman, S.M. Resnick, Longitudinal assessment of serum free testosterone concentration predicts memory performance and cognitive status in elderly men, J. Clin. Endocrnol. Metab. 87 (2002) 5001-5007.

 S.D. Moffat, A.B. Zonderman, E.J. Metter, C. Kawas, M.R. Blackman, S.M. Harman, S.M. Resnick, Free testosterone and risk for Alzheimer disease in older men, Neurology 62 (2004) 188-193.

 P.K. Molsa, R.J. Marttila, U.K. Rinne, Epidemiology of dementia in a Finnish population, Acta Neurol. Scand. 65 (1982) 541-552.

 I. Mook-Jung, I. Joo, S. Sohn, H.J. Kwon, K. Huh, M.W. Jung, Estrogen blocks neurotoxic effects of beta-amyloid (1-42) and induces neurite extension on B103 cells, Neurosci. Lett. 235 (1997) 101-104.

 G. Morali, A.E. Lemus, R. Munguia, M. Arteaga, G. Perez-Palacios, K. Sundaram, N. Kumar, C.W. Bardin, Induction of male sexual behavior in the rat by 7 alpha-methyl-19-nortestosterone, an androgen that does not undergo 5 alpha-reduction, Biol. Reprod. 49 (1993) 577-581.

 J.E. Morley, Androgens and aging, Maturitas 38 (2001) 61-71. discussion 7163.

 J.E. Morley, H.M. Perry, Androgen treatment of male hypogonadism in older males, J. Steroid Biochem. Mol. Biol. 85 (2003) 367-373.

 J.E. Morley, F.E. Kaiser, H.M. Perry, P. Patrick, P.M. Morley, P.M. Stauber, B. Vellas, R.N. Baumgartner, P.J. Garry, Longitudinal changes in testosterone, luteinizing hormone, and follicle-stimulating hormone in healthy older men, Metabol.: Clin. Exp. 46 (1997) 410-413.

 M. Muller, I. den Tonkelaar, J.H. Thijssen, D.E. Grobbee, Y.T. van der Schouw, Endogenous sex hormones in men aged 40-80 years, Eur. J. Endocrinol. 149 583-589.

 R.A. Mulnard, C.W. Cotman, C. Kawas, C.H. van Dyck, M. Sano, R. Doody, E. Koss, E. Pfeiffer, S. Jin, A. Gamst, M. Grundman, R. Thomas, L.J. Thal, Estrogen replacement therapy for treatment of mild to moderate Alzheimer disease: a randomized controlled trial, J. Am. Med. Assoc. 283 (2000) 1007.

 T.V.V. Nguyen, M. Yao, C.J. Pike, Androgens activate mitogen-activated protein kinase signaling: role in neuroprotection, J. Neurochem. 94 (2005) 1639-1651.

 S.T. Nguyen, R. Prakash, C.J. Anderson, M. Frydenberg, J.M. Haynes, Sex steroids modulate alpha 1-adrenoceptor-stimulated Ca²⁺ elevation in human cultured prostatic stromal cells, Prostate 67 (2007) 74-82.

 T.V. Nguyen, M. Yao, C.J. Pike, Flutamide and cyproterone acetate exert agonist effects: induction of androgen receptor-dependent neuroprotection, Endocrinology 148 (2007) 2936-2943.

 J. Nilsen, R.D. Brinton, Impact of progestins on estradiol potentiation of the glutamate calcium response, Neuroreport 13 (2002) 825-830.

 J. Nilsen, R.D. Brinton, Divergent impact of progesterone and medroxyprogesterone acetate (Provera) on nuclear mitogen-activated protein kinase signaling, Proc. Natl. Acad. Sci. USA 100 (2003) 10506-10511.

 J. Nilsen, R. Diaz_Brinton, Mechanism of estrogen-mediated neuroprotection: regulation of mitochondrial calcium and Bcl-2 expression, Proc. Natl. Acad. Sci. USA 100 (2003) 2842-2847.

 J. Nilsen, G. Mor, F. Naftolin, Raloxifene induces neurite outgrowth in estrogen receptor positive PC12 cells, Menopause 5 (1998) 211-216.

 J. Nilsen, S. Chen, R.D. Brinton, Dual action of estrogen on glutamate-induced calcium signaling: mechanisms requiring interaction between estrogen receptors and src/mitogen activated protein kinase pathway, Brain Res. 930 216-234.

 V.L. Nordell, M.M. Scarborough, A.K. Buchanan, F. Sohrabji, Differential effects of estrogen in the injured forebrain of young adult and reproductive senescent animals, Neurobiol. Aging 24 (2003) 733-743.

 S. Oddo, A. Caccamo, M. Kitazawa, B.P. Tseng, F.M. LaFerla, Amyloid deposition precedes tangle formation in a triple transgenic model of Alzheimer's disease, Neurobiol. Aging 24 (2003) 1063-1070.

 Effects of Estrogen or Estrogen/Progestin Regimens on Heart Disease Risk Factors in Postmenopausal Women. The Postmenopausal Estrogen/Progestin Interventions (PEPI) Trial. The Writing Group for the PEPI Trial, Jama 273 199-208.

 M. Ogiue-Ikeda, N. Tanabe, H. Mukai, Y. Hojo, G. Murakami, T. Tsurugizawa, N. Takata, T. Kimoto, S. Kawato, Rapid modulation of synaptic plasticity by estrogens as well as endocrine disrupters in hippocampal neurons, Brain Res. Rev. 57 (2008) 363-375.

 T. Ohkura, K. Isse, K. Akazawa, M. Hamamoto, Y. Yaoi, N. Hagino, Evaluation of estrogen treatment in female patients with dementia of the Alzheimer type, Endocr. J. 41 (1994) 361-371.

 A. Olariu, K. Yamada, T. Nabeshima, Amyloid pathology and protein kinase C (PKC): possible therapeutics effects of PKC activators, J. Pharmacol. Sci. 97 (2005) 1-5.

 K. O'Neill, S. Chen, R.D. Brinton, Impact of the selective estrogen receptor modulator, raloxifene, on neuronal survival and outgrowth following toxic insults associated with aging and Alzheimer's disease, Exp. Neurol. 185 63-80.

 R. Orlando, A. Caruso, G. Molinaro, M. Motolese, F. Matrisciano, G. Togna, D. Melchiorri, F. Nicoletti, V. Bruno, Nanomolar concentrations of anabolic- androgenic steroids amplify excitotoxic neuronal death in mixed mouse cortical cultures, Brain Res. 1165 (2007) 21-29.

 J. Ostrowski, J.E. Kuhns,J.A. Lupisella, M.C. Manfredi, B.C. Beehler, S.R. Krystek Jr., Y. Bi, C. Sun, R. Seethala, R. Golla, P.G. Sleph, A. Fura, Y. An, K.F. Kish, J.S. Sack, K.A. Mookhtiar, G.J. Grover, L.G. Hamann, Pharmacological and x-ray structural characterization of a novel selective androgen receptor modulator: potent hyperanabolic stimulation of skeletal muscle with hypostimulation of prostate in rats, Endocrinology 148 (2007) 4-12.

 A. Paganini-Hill, L.J. Clark, Preliminary assessment of cognitive function in breast cancer patients treated with tamoxifen, Breast Cancer Res. Treat 64 165-176.

 A. Paganini-Hill, V.W. Henderson, Estrogen deficiency and risk of Alzheimer's disease in women, Am. J. Epidemiol. 140 (1994) 256-261.

 A. Paganini-Hill, V.W. Henderson, Estrogen replacement therapy and risk of Alzheimer disease, Arch. Intern. Med. 156 (1996) 2213-2217.

 A.M. Paoletti, S. Congia, S. Lello, D. Tedde, M. Orru, M. Pistis, M. Pilloni, P. Zedda, A. Loddo, G.B. Melis, Low androgenization index in elderly women and elderly men with Alzheimer's disease, Neurology 62 (2004) 301-303.

 S.C. Papasozomenos, The heat shock-induced hyperphosphorylation of tau is estrogen-independent and prevented by androgens: implications for Alzheimer disease, Proc. Natl. Acad. Sci. USA 94 (1997) 6612-6617.

 S.C. Papasozomenos, T. Papasozomenos, Androgens prevent the heat shock- induced hyperphosphorylation but not dephosphorylation of t in female rats. implications for Alzheimer's disease, J. Alzheimers Dis. 1 (1999) 147153.

 S. Papasozomenos, A. Shanavas, Testosterone prevents the heat shock- induced overactivation of glycogen synthase kinase-3 beta but not of cyclin- dependent kinase 5 and c-Jun NH2-terminal kinase and concomitantly abolishes hyperphosphorylation of tau: implications for Alzheimer's disease, Proc. Natl. Acad. Sci. USA 99 (2002) 1140-1145.

 S.Y. Park, C. Tournell, R.C. Sinjoanu, A. Ferreira, Caspase-3- and calpain- mediated tau cleavage are differentially prevented by estrogen and testosterone in beta-amyloid-treated hippocampal neurons, Neuroscience 144 (2007) 119-127.

 C. Patrone, S. Andersson, L. Korhonen, D. Lindholm, Estrogen receptor- dependent regulation of sensory neuron survival in developing dorsal root ganglion, Proc. Natl. Acad. Sci. USA 96 (1999) 10905-10910.

 C. Pennanen, M. Kivipelto, S. Tuomainen, P. Hartikainen, T. Hanninen, M.P. Laakso, M. Hallikainen, M. Vanhanen, A. Nissinen, E.L. Helkala, P. Vainio, R. Vanninen, K. Partanen, H. Soininen, Hippocampus and entorhinal cortex in mild cognitive impairment and early AD, Neurobiol. Aging 25 (2004) 303310.

 B. Perillo, A. Sasso, C. Abbondanza, G. Palumbo, 17beta-estradiol inhibits apoptosis in MCF-7 cells, inducing bcl-2 expression via two estrogen- responsive elements present in the coding sequence, Mol. Cell Biol. 20 2890-2901.

 I. Persson, H.O. Adami, L. Bergkvist, A. Lindgren, B. Pettersson, R. Hoover, C. Schairer, Risk of endometrial cancer after treatment with oestrogens alone or in conjunction with progestogens: results of a prospective study, Bmj 298 (1989) 147-151.

 S.S. Petanceska, V. Nagy, D. Frail, S. Gandy, Ovariectomy and 17beta-estradiol modulate the levels of Alzheimer's amyloid beta peptides in brain, Exp. Gerontol. 35 (2000) 1317-1325.

 C.J. Pike, Estrogen modulates neuronal Bcl-x_L expression and beta-amyloid- induced apoptosis: relevance to Alzheimer's disease, J. Neurochem. 72 (1999) 1552-1563.

  C.J. Pike, Testosterone attenuates beta-amyloid toxicity in cultured hippocampal neurons, Brain Res. 919 (2001) 160-165.

 C.J. Pike, A.J. Walencewicz, C.G. Glabe, C.W. Cotman, Aggregation-related toxicity of synthetic beta-amyloid protein in hippocampal cultures, Eur. J. Pharmacol. 207 (1991) 367-368.

 C.J. Pike, B.J. Cummings, C.W. Cotman, beta-Amyloid induces neuritic dystrophy in vitro: similarities with Alzheimer pathology, Neuroreport 3 (1992) 769-772.

  C.J. Pike, D. Burdick, A.J. Walencewicz, C.G. Glabe, C.W. Cotman, Neurodegeneration induced by beta-amyloid peptides in vitro: the role of peptide assembly state, J. Neurosci. 13 (1993) 1676-1687.

 C.J. Pike, B.J. Cummings, R. Monzavi, C.W. Cotman, Beta-amyloid-induced changes in cultured astrocytes parallel reactive astrocytosis associated with senile plaques in Alzheimer's disease, Neuroscience 63 (1994) 517531.

 J.V. Pinkerton, V.W. Henderson, Estrogen and cognition, with a focus on Alzheimer's disease, Semin. Reprod. Med. 23 (2005) 172-179.

 P. Polo-Kantola, R. Portin, O. Polo, H. Helenius, K. Irjala, R. Erkkola, The effect of short-term estrogen replacement therapy on cognition: a randomized, double-blind, cross-over trial in postmenopausal women, Obstet. Gynecol. 91

 (1998)459-466.

 D.V. Pow, V.H. Perry, J.F. Morris, S. Gordon, Microglia in the neurohypophysis associate with and endocytose terminal portions of neurosecretory neurons, Neuroscience 33 (1989) 567-578.

 J. Qiu, M.A. Bosch, S.C. Tobias, D.K. Grandy, T.S. Scanlan, O.K. Ronnekleiv, M.J. Kelly, Rapid signaling of estrogen in hypothalamic neurons involves a novel G-protein-coupled estrogen receptor that activates protein kinase C, J. Neurosci. 23 (2003) 9529-9540.

 J. Raber, Androgens, apoE, and Alzheimer's disease, Sci. Aging Knowledge Environ. 2004 (2004) re2.

 J. Raber, AR, apoE, and cognitive function, Horm. Behav. 53 (2008) 706-715.

 M. Ramsden, A.C. Nyborg, M.P. Murphy, L. Chang, F.Z. Stanczyk, T.E. Golde, C.J. Pike, Androgens modulate beta-amyloid levels in male rat brain, J. Neurochem. 87 (2003) 1052-1055.

 M. Ramsden, T.M. Shin, C.J. Pike, Androgens modulate neuronal vulnerability to kainate lesion, Neuroscience 122 (2003) 573-578.

 S.R. Rapp, M.A. Espeland, S.A. Shumaker, V.W. Henderson, R.L. Brunner, J.E. Manson, M.L. Gass, M.L. Stefanick, D.S. Lane, J. Hays, K.C. Johnson, L.H. Coker, M. Dailey, D. Bowen, Effect of estrogen plus progestin on global cognitive function in postmenopausal women: the Women's Health Initiative Memory Study: a randomized controlled trial, Jama: J. Am. Med. Assoc. 289 (2003) 2663-2672.

 S. Rasmuson, B. Nasman, K. Carlstrom, T. Olsson, Increased levels of adrenocortical and gonadal hormones in mild to moderate Alzheimer's disease, Dement. Geriatr. Cogn. Disord. 13 (2002) 74-79.

 R.F. Regan, Y. Guo, Estrogens attenuate neuronal injury due to hemoglobin, chemical hypoxia, and excitatory amino acids in murine cortical cultures, Brain Res. 764 (1997) 133-140.

 S.M. Resnick, V.W. Henderson, Hormone therapy and risk of Alzheimer disease: a critical time, Jama 288 (2002) 2170-2172.

 M.E. Rhodes, C.A. Frye, Androgens in the hippocampus can alter, and be altered by, ictal activity, Pharmacol. Biochem. Behav. 78 (2004) 483-493.

 C.A. Rice-Evans, N.J. Miller, G. Paganga, Structure-antioxidant activity relationships of flavonoids and phenolic acids, Free Rad. Biol. Med. 20 933-956.

 C. Rice-Evans, N. Miller, G. Paganga, Antioxidant properties of phenolic compounds, Trends Plant Sci. 2 (1997) 152-159.

 P. Riederer, S. Hoyer, From benefit to damage. Glutamate and advanced glycation end products in Alzheimer brain, J. Neural. Transm. 113 (2006) 1671-1677.

 W.A. Rocca, L.A. Amaducci, B.S. Schoenberg, Epidemiology of clinically diagnosed Alzheimer's disease, Ann. Neurol. 19 (1986) 415-424.

 J. Rogers, R. Strohmeyer, C. Kovelowski, R.G. Li, Microglia and inflammatory mechanisms in the clearance of amyloid beta peptide, Glia 40 (2002) 260269.

 E.R. Rosario, C.J. Pike, Androgen regulation of beta-amyloid protein and the risk of Alzheimer's disease, Brain Res. Rev. 57 (2008) 444-453.

 E.R. Rosario, L. Chang, F.Z. Stanczyk, C.J. Pike, Age-related testosterone depletion and the development of Alzheimer disease, Jama 292 (2004) 14311432.

 E.R. Rosario, J.C. Carroll, S. Oddo, F.M. LaFerla, C.J. Pike, Androgens regulate the development of neuropathology in a triple transgenic mouse model of Alzheimer's disease, J. Neurosci. 26 (2006) 13384-13389.

 E.R. Rosario, M. Ramsden, C.J. Pike, Progestins inhibit the neuroprotective effects of estrogen in rat hippocampus, Brain Res. 1099 (2006) 206-210.

 E.R. Rosario, L. Chang, E.H. Head, F. Z. Stanczyk, C. J. Pike, Brain levels of sex steroid hormones in men and women during normal aging and in Alzheimer's disease, Neurobiol. Aging, in press. M.J. Rowan, I. Klyubin, Q. Wang, N.W. Hu, R. Anwyl, Synaptic memory mechanisms: Alzheimer's disease amyloid beta-peptide-induced dysfunction, Biochem. Soc. Trans. 35 (2007) 1219-1223.

 B.S. Rubin, T.O. Fox, R.S. Bridges, Estrogen binding in nuclear and cytosolic extracts from brain and pituitary of middle-aged female rats, Brain Res. 383 (1986) 60-67.

 A. Ruitenberg, A. Ott, J.C. van Swieten, A. Hofman, M.M.B. Breteler, Incidence of dementia: does gender make a difference?, Neurobiol Aging 22 (2001) 575-580.

 M. Sastre, J. Walter, S.M. Gentleman, Interactions between APP secretases and inflammatory mediators, J. Neuroinflammation 5 (2008) 25.

 E. Savaskan, G. Olivieri, F. Meier, R. Ravid, F. Muller-Spahn, Hippocampal estrogen beta-receptor immunoreactivity is increased in Alzheimer's disease, Brain Res. 908 (2001) 113-119.

 C. Schultz, H. Braak, E. Braak, A sex difference in neurodegeneration of the human hypothalamus, Neurosci. Lett. 212 (1996) 103-106.

 C. Schultz, E. Ghebremedhin, E. Braak, H. Braak, Sex-dependent cytoskeletal changes of the human hypothalamus develop independently of Alzheimer's disease, Exp. Neurol. 160 (1999) 186-193.

 M. Schumacher, R. Sitruk-Ware, A.F. De Nicola, Progesterone and progestins: neuroprotection and myelin repair, Curr. Opin. Pharmacol. 8 (2008) 740-746.

 N. Schupf, J.H. Lee, M. Wei, D. Pang, C. Chace, R. Cheng, W.B. Zigman, B. Tycko, W. Silverman, Estrogen receptor-alpha variants increase risk of Alzheimer's disease in women with Down syndrome, Dement. Geriatr. Cogn. Disord. 25

 476-482.

 D.J. Selkoe, T. Yamazaki, M. Citron, M.B. Podlisny, E.H. Koo, D.B. Teplow, C. Haass, The role of APP processing and trafficking pathways in the formation of amyloid beta-protein, Ann. NY Acad. Sci. 777 (1996) 57-64.

 A. Selvamani, F. Sohrabji, Reproductive age modulates the impact of focal ischemia on the forebrain as well as the effects of estrogen treatment in female rats, Neurobiol. Aging (2008).

 G. Sétâlô, M. Singh, I.S. Nethrapalli, C.D. Toran-Allerand, Protein kinase C activity is necessary for estrogen-induced Erk phosphorylation in neocortical explants, J. Neurochem. Res. 30 (2005) 779-79o.

 Y. Shang, M. Brown, Molecular determinants for the tissue specificity of SERMs, Science 295 (2002) 2465-2468.

 T.C. Shao, H.L. Li, S. Kasper, R. Matusik, M. Ittmann, G.R. Cunningham, Comparison of the growth-promoting effects of testosterone and 7-alpha- methyl-19-nor-testosterone (MENT) on the prostate and levator ani muscle of LPB-tag transgenic mice, Prostate 66 (2006) 369-376.

 M. Sheffield-Moore, R.J. Urban, An overview of the endocrinology of skeletal muscle, Trends Endocrinol. Metab. 15 (2004) 110-115.

 L.C. Sheldahl, L.K. Marriott, D.M. Bryant, R.A. Shapiro, D.M. Dorsa, Neuroprotective effects of estrogen and selective estrogen receptor modulators begin at the plasma membrane, Minerva Endocrinol. 32 (2007) 87-94.

 R. Shen, M. Sumitomo, J. Dai, D.O. Hardy, D. Navarro, B. Usmani, C.N. Papandreou, L.B. Hersh, M.A. Shipp, L.P. Freedman, D.M. Nanus, Identification and characterization of two androgen response regions in the human neutral endopeptidase gene, Mol. Cell Endocrinol. 170 (2000) 131-142.

 B.B. Sherwin, Estrogen and/or androgen replacement therapy and cognitive functioning in surgically menopausal women, Psychoneuroendocrinology 13 (1988) 345-357.

 B.B. Sherwin, Estrogen and cognitive functioning in women, Endocr. Rev. 24 133-151.

 J. Shi, K.S. Panickar, S.-H. Yang, O. Rabbani, A.L. Day, J.W. Simpkins, Estrogen attenuates over-expression of b-amyloid precursor protein messager RNA in an animal model of focal ischemia, Brain Res. 810 (1998) 87-92.

 V. Shilling, V. Jenkins, L. Fallowfield, T. Howell, The effects of hormone therapy on cognition in breast cancer, J. Steroid Biochem. Mol. Biol. 86 (2003) 405-412.

 P.J. Shughrue, I. Merchenthaler, Estrogen is more than just a ‘‘Sex Hormone”: novel sites for estrogen action in the hippocampus and cerebral cortex, Front. Neuroendocrinol. 21 (2000) 95-101.

 P.J. Shughrue, M.V. Lane, I. Merchenthaler, Comparative distribution of estrogen receptor-alpha and -beta mRNA in the rat central nervous system, J. Comp. Neurol. 388 (1997) 507-525.

  S.A. Shumaker, C. Legault, S.R. Rapp, L. Thal, R.B. Wallace, J.K. Ockene, S.L. Hendrix, B.N. Jones, A.R. Assaf, R.D. Jackson, J.M. Kotchen, S. Wassertheil_Smoller, J. Wactawski_Wende, Estrogen plus progestin and the incidence of dementia and mild cognitive impairment in postmenopausal women: the Women's Health Initiative Memory Study: a randomized controlled trial, Jama: J. Am. Med. Assoc. 289 (2003) 2651-2662.

 S.A. Shumaker, C. Legault, L. Kuller, S.R. Rapp, L. Thal, D.S. Lane, H. Fillit, M.L. Stefanick, S.L. Hendrix, C.E. Lewis, K. Masaki, L.H. Coker, Conjugated equine estrogens and incidence of probable dementia and mild cognitive impairment in postmenopausal women: Women's Health Initiative Memory Study, Jama 291 (2004) 2947-2958.

 I. Silva, L.E. Mello, E. Freymuller, M.A. Haidar, E.C. Baracat, Estrogen, progestogen and tamoxifen increase synaptic density of the hippocampus of ovariectomized rats, Neurosci. Lett. 291 (2000) 183-186.

 R.B. Simerly, C. Chang, M. Muramatsu, L.W. Swanson, Distribution of androgen and estrogen receptor mRNA-containing cells in the rat brain: an in situ hybridization study, J. Comp. Neurol. 294 (1990) 76-95.

 C.A. Singer, K.L. Rogers, T.M. Strickland, D.M. Dorsa, Estrogen protects primary cortical neurons from glutamate toxicity, Neurosci. Lett. 212 (1996) 13-16.

 C.A. Singer, K.L. Rogers, D.M. Dorsa, Modulation of Bcl-2 expression: a potential component of estrogen protection in NT2 neurons, Neuroreport 9 (1998) 2565-2568.

 C.A. Singer, X.A. Figueroa_Masot, R.H. Batchelor, D.M. Dorsa, The mitogen- activated protein kinase pathway mediates estrogen neuroprotection after glutamate toxicity in primary cortical neurons, J. Neurosci.: Official J. Soc. Neurosci. 19 (1999) 2455-2463.

 M. Singh, Ovarian hormones elicit phosphorylation of Akt and extracellularsignal regulated kinase in explants of the cerebral cortex, Endocrine 14 407-415.

 M. Singh, G. Setalo Jr., X. Guan, M. Warren, C.D. Toran-Allerand, Estrogen- induced activation of mitogen-activated protein kinase in cerebral cortical explants: convergence of estrogen and neurotrophin signaling pathways, J. Neurosci. 19 (1999) 1179-1188.

 S.E. Stoltzner, N.C. Berchtold, C.W. Cotman, C.J. Pike, Estrogen regulates Bcl-x expression in rat hippocampus, Neuroreport 12 (2001) 2797-2800.

 D.J. Stone, I. Rozovsky, T.E. Morgan, C.P. Anderson, L.M. Lopez, J. Shick, C.E. Finch, Effects of age on gene expression during estrogen-induced synaptic sprouting in the female rat, Exp. Neurol. 165 (2000) 46-57.

 W.J. Strittmatter, A.D. Roses, Apolipoprotein E and Alzheimer disease, Proc. Natl. Acad. Sci. USA 92 (1995) 4725-4727.

 R.G. Struble, B.P. Nathan, C. Cady, X. Cheng, M. McAsey, Estradiol regulation of astroglia and apolipoprotein E: an important role in neuronal regeneration, Exp. Gerontol. 42 (2007) 54-63.

 M.T. Subbiah, B. Kessel, M. Agrawal, R. Rajan, W. Abplanalp, Z. Rymaszewski, Antioxidant potential of specific estrogens on lipid peroxidation, J. Clin. Endocrinol. Metab. 77 (1993) 1095-1097.

 K. Sugioka, Y. Shimosegawa, M. Nakano, Estrogens as natural antioxidants of membrane phospholipid peroxidation, FEBS Lett. 210 (1987) 37-39.

 R.S. Swerdloff, C. Wang, Androgen deficiency and aging in men, Western J. Med. 159 (1993) 579-585.

 R.S. Tan, J.W. Culberson, An integrative review on current evidence of testosterone replacement therapy for the andropause, Maturitas 45 (2003) 15-27.

 R.S. Tan, S.J. Pu, A pilot study on the effects of testosterone in hypogonadal aging male patients with Alzheimer's disease, Aging Male 6 (2003) 13.

 M.X. Tang, D. Jacobs, Y. Stern, K. Marder, P.R. Schofield, B. Gurland, H. Andrews, R. Mayeux, Effect of oestrogen during menopause on risk and age at onset of Alzheimer's disease, The Lancet 348 (1996) 429.

 L. Tanzer, K.J. Jones, Gonadal steroid regulation of hamster facial nerve regeneration: effects of dihydrotestosterone and estradiol, Exp. Neurol. 146 258-264.

 R.E. Tanzi, R.D. Moir, S.L. Wagner, Clearance of Alzheimer's A[beta] Peptide: the many roads to perdition, Neuron 43 (2004) 605-608.

 S.H. Tariq, M.T. Haren, M.J. Kim, J.E. Morley, Andropause: is the emperor wearing any clothes?, Rev Endocr. Metab. Disord. 6 (2005) 77-84.

 M.K. Thakur, S.T. Mani, Estradiol regulates APP mRNA alternative splicing in the mice brain cortex, Neurosci. Lett. 381 (2005) 154-157.

 T. Thomas, M. Bryant, L. Clark, A. Garces, J. Rhodin, Estrogen and raloxifene activities on amyloid - induced inflammatory reaction, Microvasc. Res. 61

 28-39.

 C.D. Toran-Allerand, M. Singh, G. Setalo, Novel mechanisms of estrogen action in the brain: new players in an old story, Front. Neuroendocrinol. 20 (1999) 97-121.

 P.R. Turner, K. O'Connor, W.P. Tate, W.C. Abraham, Roles of amyloid precursor protein and its fragments in regulating neural activity, plasticity and memory, Prog. Neurobiol. 70 (2003) 1-32.

 E.R.L.C. Vardy, A.J. Catto, N.M. Hooper, Proteolytic mechanisms in amyloid- [beta] metabolism: therapeutic implications for Alzheimer's disease, Trends Mol. Med. 11 (2005) 464-472.

 S. Vasto, G. Candore, F. Listi, C.R. Balistreri, G. Colonna-Romano, M. Malavolta, D. Lio, D. Nuzzo, E. Mocchegiani, D. Di Bona, C. Caruso, Inflammation, genes and zinc in Alzheimer's disease, Brain Res. Rev. 58 (2008) 96-105.

 C. Vaughan, F.C. Goldstein, J.L. Tenover, Exogenous testosterone alone or with finasteride does not improve measurements of cognition in healthy older men with low serum testosterone, J. Androl. 28 (2007) 875-882.

 H. Vedder, N. Anthes, G. Stumm, C. Wurz, C. Behl, J.-C. Krieg, Estrogen hormones reduce lipid peroxidation in cells and tissues of the central nervous system, J. Neurochem. 72 (1999) 2531-2538.

 E. Vegeto, C. Bonincontro, G. Pollio, A. Sala, S. Viappiani, F. Nardi, A. Brusadelli, B. Viviani, P. Ciana, A. Maggi, Estrogen prevents the lipopolysaccharide- induced inflammatory response in microglia, J. Neurosci. 21 (2001) 18091818.

 K. Venken, S. Boonen, E. Van Herck, L. Vandenput, N. Kumar, R. Sitruk-Ware, K. Sundaram, R. Bouillon, D. Vanderschueren, Bone and muscle protective potential of the prostate-sparing synthetic androgen 7alpha-methyl-19- nortestosterone: evidence from the aged orchidectomized male rat model, Bone 36 (2005) 663-670.

 G. Verdile, S. Fuller, C.S. Atwood, S.M. Laws, S.E. Gandy, R.N. Martins, The role of beta amyloid in Alzheimer's disease: still a cause of everything or the only one who got caught?, Pharmacol Res. 50 (2004) 397-409.

 B. Vincent, J.D. Smith, Effect of estradiol on neuronal Swedish-mutated betaamyloid precursor protein metabolism: reversal by astrocytic cells, Biochem. Biophys/Res. Commun. 271 (2000) 82-85.

 S. von Eckardstein, G. Noe, V. Brache, E. Nieschlag, H. Croxatto, F. Alvarez, A. Moo-Young, I. Sivin, N. Kumar, M. Small, K. Sundaram, A clinical trial of 7 alpha-methyl-19-nortestosterone implants for possible use as a long-acting contraceptive for men, J. Clin Endocrinol. Metab. 88 (2003) 5232-5239.

 E.J. Wahjoepramono, L.K. Wijaya, K. Taddei, G. Martins, M. Howard, K.d. Ruyck, K. Bates, S.S. Dhaliwald, G. Verdile, M. Carruthers, R.N. Martins, Distinct effects of testosterone on plasma and cerebrospinal fluid amyloid-b levels, J. Alzheimer's Dis. 129 (2008) 129-137.

 D. Walsh, D. Selkoe, A beta oligomers - a decade of discovery, J. Neurochem. 101 (2007) 1172-1184.

 D.M. Walsh, I. Klyubin, J.V. Fadeeva, M.J. Rowan, D.J. Selkoe, Amyloid-beta oligomers: their production, toxicity and therapeutic inhibition, Biochem. Soc. Trans. 30 (2002) 552-557.

 P.N. Wang, S.Q. Liao, R.S. Liu, C.Y. Liu, H.T. Chao, S.R. Lu, H.Y. Yu, S.J. Wang, H.C. Liu, Effects of estrogen on cognition, mood, and cerebral blood flow in AD: a controlled study, Neurology 54 (2000) 2061-2066.

 J. Wang, H. Tanila, J. Puolivali, I. Kadish, T. van Groen, Gender differences in the amount and deposition of amyloidbeta in APP_swe and PS1 double transgenic mice, Neurobiol. Dis. 14 (2003) 318-327.

 S.C. Waring, W.A. Rocca, R.C. Petersen, P.C. O_Brien, E.G. Tangalos, E. Kokmen, Postmenopausal estrogen replacement therapy and risk of AD: a population- based study, Neurology 52 (1999) 965-970.

 T. Watanabe, S. Koba, M. Kawamura, M. Itokawa, T. Idei, Y. Nakagawa, T. Iguchi, T. Katagiri, Small dense low-density lipoprotein and carotid atherosclerosis in relation to vascular dementia, Metabolism 53 (2004) 476-482.

 J.J. Watters, J.S. Campbell, M.J. Cunningham, E.G. Krebs, D.M. Dorsa, Rapid membrane effects of steroids in neuroblastoma cells: effects of estrogen on mitogen activated protein kinase signalling cascade and c-fos immediate early gene transcription, Endocrinology 138 (1997) 4030-4033.

 C.E. Weaver Jr., M. Park-Chung, T.T. Gibbs, D.H. Farb, 17beta-Estradiol protects against NMDA-induced excitotoxicity by direct inhibition of NMDA receptors, Brain Res. 761 (1997) 338-341.

 C.L. Weaver, M. Espinoza, Y. Kress, P. Davies, Conformational change as one of the earliest alterations of tau in Alzheimer's disease, Neurobiol. Aging 21 (2000) 719-727.

 J.H. Weiss, C.J. Pike, C.W. Cotman, Ca²⁺ channel blockers attenuate betaamyloid peptide toxicity to cortical neurons in culture, J. Neurochem. 62 (1994) 372-375.

 W. Wharton, E. Brinton, M. Cedars, R.A. Lobo, J. Manson, G.R. Merriam, V.M. Miller, N. Santoro, H.S. Taylor, S.M. Harman, S. Asthana, Baseline cognitive and demographic characteristics of women enrolled in the Kronos Early Estrogen Prevention Study (KEEPS) Alzheimer's & Dementia, J. Alzheimer's Assoc. 4 (2008) T792.

 K.C. Wilhelmsen, L.N. Clark, B.L. Miller, D.H. Geschwind, Tau mutations in frontotemporal dementia, Dement. Geriatr. Cogn. Disord. 10 (Suppl. 1) (1999) 88-92.

 E.M. Wilson, Muscle-bound? A tissue-selective nonsteroidal androgen receptor modulator, Endocrinology 148 (2007) 1-3.

 P.M. Wise, Estrogen therapy: does it help or hurt the adult and aging brain? Insights derived from animal models, Neuroscience 138 (2006) 831-835.

 P.M. Wise, B. Parsons, Nuclear estradiol and cytosol progestin receptor concentrations in the brain and the pituitary gland and sexual behavior in ovariectomized estradiol-treated middle-aged rats, Endocrinology 115 (1984) 810-816.

 C.S. Woolley, Bruce S. McEwen, Roles of estradiol and progesterone in regulation of hippocampal dendritic spine density during the estrous cycle in the rat, J. Comp. Neurol. 336 (1993) 293-306.

 X. Wu, M.A. Glinn, N.L. Ostrowski, Y. Su, B. Ni, H.W. Cole, H.U. Bryant, S.M. Paul, Raloxifene and estradiol benzoate both fully restore hippocampal choline acetyltransferase activity in ovariectomized rats, Brain Res. 847 (1999) 98-104.

 T.W. Wu, J.M. Wang, S. Chen, R.D. Brinton, 17Beta-estradiol induced Ca²⁺ influx via L-type calcium channels activates the Src/ERK/cyclic- AMP response element binding protein signal pathway and BCL-2 expression in rat hippocampal neurons: a potential initiation mechanism for estrogen-induced neuroprotection, Neuroscience 135 59-72.

 Z.-M. Xiao, L. Sun, Y.-M. Liu, J.-J. Zhang, J. Huang, Estrogen regulation of the neprilysin gene through a hormone-responsive element, J. Mol. Neurosci., in press.

 H. Xu, G.K. Gouras, J.P. Greenfield, B. Vincent, J. Naslund, L. Mazzarelli, G. Fried, J.N. Jovanovic, M. Seeger, N.R. Relkin, F. Liao, F. Checler, J.D. Buxbaum, B.T. Chait, G. Thinakaran, S.S. Sisodia, R. Wang, P. Greengard, S. Gandy, Estrogen reduces neuronal generation of Alzheimer beta-amyloid peptides, Nat. Med. 4 (1998) 447-451.

 Y. Xu, S.J. Armstrong, I.A. Arenas, D.J. Pehowich, S.T. Davidge, Cardioprotection by chronic estrogen or superoxide dismutase mimetic treatment in the aged female rat, Am. J. Physiol. Heart Circ. Physiol. 287

 H165-H171.

 H. Xu, R. Wang, Y.-W. Zhang, X. Zhang, Estrogen, -amyloid metabolism/ trafficking, and Alzheimer's disease, Ann. NY Acad. Sci. 1089 (2006) 324-342.

 K. Yaffe, K. Krueger, S. Sarkar, D. Grady, E. Barrett-Connor, D.A. Cox, T. Nickelsen, The multiple outcomes of raloxifene evaluation investigators, cognitive function in postmenopausal women treated with raloxifene, New Engl. J. Med. 344 (2001) 1207-1213.

 K. Yaffe, K. Krueger, S.R. Cummings, T. Blackwell, V.W. Henderson, S. Sarkar, K. Ensrud, D. Grady, Effect of raloxifene on prevention of dementia and cognitive impairment in older women: the Multiple Outcomes of Raloxifene Evaluation (MORE) randomized trial, Am. J. Psychiatry 162 (2005) 683690.

 S.H. Yang, E. Perez, J. Cutright, R. Liu, Z. He, A.L. Day, J.W. Simpkins, Testosterone increases neurotoxicity of glutamate in vitro and ischemia- reperfusion injury in an animal model, J. Appl. Physiol. 92 (2002) 195-201.

 S.H. Yang, R. Liu, Y. Wen, E. Perez, J. Cutright, A.M. Brun-Zinkernagel, M. Singh, A.L. Day, J.W. Simpkins, Neuroendocrine mechanism for tolerance to cerebral ischemia-reperfusion injury in male rats, J. Neurobiol. 62 (2005) 341-351.

 B.A. Yankner, T. Lu, Amyloid {beta}-Protein Toxicity and the Pathogenesis of Alzheimer Disease, J. Biol. Chem. 284 (2009) 4755-4759.

 M. Yao, T.V. Nguyen, C.J. Pike, Beta-amyloid-induced neuronal apoptosis involves c-Jun N-terminal kinase-dependent downregulation of Bcl-w, J. Neurosci. 25 (2005) 1149-1158.

 M. Yao, T.V. Nguyen, C.J. Pike, Estrogen regulates Bcl-w and Bim expression: role in protection against beta-amyloid peptide-induced neuronal death, J. Neurosci. 27 (2007) 1422-1433.

 M. Yao, T.V. Nguyen, E.R. Rosario, M. Ramsden, C.J. Pike, Androgens regulate neprilysin expression: role in reducing beta-amyloid levels, J. Neurochem. (2008).

 W.H. Yu, Effect of testosterone on the regeneration of the hypoglossal nerve in rats, Exp. Neurol. 77 (1982) 129-141.

 W.H. Yu, Administration of testosterone attenuates neuronal loss following axotomy in the brain-stem motor nuclei of female rats, J. Neurosci. 9 (1989) 3908-3914.

 W.H. Yu, R. Srinivasan, Effect of testosterone and 5 alphadihydrotestosterone on regeneration of the hypoglossal nerve in rats, Exp. Neurol. 71 (1981) 431-435.

 X. Yue, M. Lu, T. Lancaster, P. Cao, S.-I. Honda, M. Staufenbiel, N. Harada, Z. Zhong, Y. Shen, R. Li, Brain estrogen deficiency accelerates Ab plaque formation in an Alzheimer's disease animal model, Proc. Natl. Acad. Sci. 102 (2005) 19198-19203.

 T.Y. Yune, H.G. Park, J.Y. Lee, T.H. Oh, Estrogen-induced Bcl-2 expression after spinal cord injury is mediated through phosphoinositide-3-kinase/Akt- dependent CREB activation, J. Neurotrauma. 25 (2008) 1121-1131.

 P.P. Zandi, M.C. Carlson, B.L. Plassman, K.A. Welsh-Bohmer, L.S. Mayer, D.C. Steffens, J.C.S. Breitner, Hormone replacement therapy and incidence of Alzheimer disease in older women: the Cache County Study, J. Am. Med. Assoc. 288 (2002) 2123-2129.

 L. Zhang, D.R.C.A. Rubinow, G.-q. Xaing, B.-S. Li, Y.H. Chang, D. Maric, J.L. Barker, W. Ma, Estrogen protects against b-amyloid-induced neurotoxicity in rat hippocampal neurons by activation of Akt, Neuroreport 12 (2001) 19191923.

 Y. Zhang, N. Champagne, L.K. Beitel, C.G. Goodyer, M. Trifiro, A. LeBlanc, Estrogen and androgen protection of human neurons against intracellular amyloid beta(1-42) toxicity through heat shock protein 70, J. Neurosci. 24 5315-5321.

 S. Zhang, Y. Huang, Y.C. Zhu, T. Yao, Estrogen stimulates release of secreted amyloid precursor protein from primary rat cortical neurons via protein kinase C pathway, Acta Pharmacol. Sin. 26 (2005) 171-176.

 Q.G. Zhang, R. Wang, M. Khan, V. Mahesh, D.W. Brann, Role of Dickkopf-1, an antagonist of the Wnt/beta-catenin signaling pathway, in estrogen-induced neuroprotection and attenuation of tau phosphorylation, J. Neurosci. 28 (2008) 8430-8441.

 L. Zhao, R.D. Brinton, Estrogen receptor alpha and beta differentially regulate intracellular Ca(2+) dynamics leading to ERK phosphorylation and estrogen neuroprotection in hippocampal neurons, Brain Res. 1172 (2007) 48-59.

 L. Zhao, T.W. Wu, R.D. Brinton, Estrogen receptor subtypes alpha and beta contribute to neuroprotection and increased Bcl-2 expression in primary hippocampal neurons, Brain Res. 1010 (2004) 22-34.

 L. Zhao, C. Jin, Z. Mao, M.B. Gopinathan, K. Rehder, R.D. Brinton, Design, synthesis, and estrogenic activity of a novel estrogen receptor modulator - a hybrid structure of 17beta-estradiol and vitamin E in hippocampal neurons, J. Med. Chem. 50 (2007) 4471-4481.

 H. Zheng, H. Xu, S.N. Uljon, R. Gross, K. Hardy, J. Gaynor, J. Lafrancois, J. Simpkins, L.M. Refolo, S. Petanceska, R. Wang, K. Duff, Modulation of A(beta) peptides by estrogen in mouse models, J. Neurochem. 80 (2002) 191196.